血清CTRP1、CTRP5和CTRP1/CTRP5水平与不稳定型心绞痛的关系

Relationship between serum CTRP1,CTRP5 and CTRP1/CTRP5 levels and unstable angina pectoris

目的探讨血清补体C1q/肿瘤坏死因子相关蛋白1(CTRP1)、CTRP5及CTRP1与CTRP5的比值(CTRP1/CTRP5)水平与不稳定型心绞痛(UAP)的关系。方法以103例UAP患者为研究对象,根据Gensini(GS)积分的中位数将其分为冠状动脉轻度狭窄组(GS≤39分,53例)和冠状动脉重度狭窄组(GS>39分,50例);根据冠状动脉病变支数将其分为单支病变组(20例)、双支病变组(46例)和多支病变组(37例)。选取同期冠状动脉造影结果显示冠状动脉狭窄程度<50%的47例心脏神经官能症患者作为对照组。采用酶联免疫吸附试验(ELISA)检测血清CTRP1和CTRP5的水平,并计算CTRP1/CTRP5比值。结果UAP组血清CTRP1、CTRP5和CTRP1/CTRP5水平高于对照组(P<0.05)。重度狭窄组血清CTRP1和CTRP5水平高于轻度狭窄组(P<0.05)。在不同冠状动脉病变支数的UAP中,多支病变组血清CTRP1水平高于单支病变组(P<0.05)。Logistic回归结果显示,CTRP1水平升高是UAP发生的独立危险因素。ROC曲线分析显示血清CTRP1、CTRP5和CTRP1/CTRP5辅助筛查UAP的ROC曲线下面积(AUC)分别为0.757、0.631和0.625,cut-off值分别为870.33μg/L、43.04μg/L和18.41,CTRP1/CTRP5的敏感度较高(84.5%),CTRP1的特异度较高(78.7%)。结论CTRP1和CTRP5参与了UAP的发生和发展过程,检测血清CTRP1和CTRP5并计算CTRP1/CTRP5的水平有助于UAP的筛查和病情评估。

Objective To investigate the relationship between serum complement-Clq/TNF related protein 1(CTRP1),CTRP5 and ratio of CTRP1 to CTRP5(CTRP1/CTRP5)and unstable angina pectoris(UAP).Methods A total of 103 patients with UAP were selected as subjects.Patients were divided into the mild coronary stenosis group(GS≤39,n=53)and the severe coronary stenosis group(GS>39,n=50)according to the median of Gensini(GS)score.Patients were divided into the single-vessel lesion group(n=20),the double-vessel lesion group(n=46)and the multi-vessel lesion group(n=37),according to the number of coronary artery lesions.Forty-seven patients with cardiac neurosis whose coronary stenosis was less than 50%were selected as the control group.Enzyme linked immunosorbent assay was used to detect serum levels of CTRP1 and CTRP5,and then the level of CTRP1/CTRP5 was calculated.Results The levels of CTRP1,CTRP5 and CTRP1/CTRP5 were higher in the UAP group than those in the control group(P<0.05).The levels of CTRP1 and CTRP5were higher in the severe coronary stenosis group than those of the mild coronary stenosis group(P<0.05).The level of CTRP1 was higher in the multi-vessel lesion group than that in the single-vessel lesion group among UAP with different vascular lesions(P<0.05).Results of multivariate Logistic analysis showed that CTRP1 was an independent predictor of UAP.ROC curve analysis showed that the area under the ROC curve(AUC)of serum CTRP1,CTRP5 and CTRP1/CTRP5 for UAP assisted screening were 0.757,0.631 and 0.625,and the cut-off values were 870.33μg/L,43.04μg/L and 18.41,respectively.The sensitivity of CTRP1/CTRP5 was higher(84.5%),and the specificity of CTRP1 was higher(78.7%).Conclusion CTRP1 and CTRP5 are involved in the occurrence and development of UAP.Detection of serum levels of CTRP1 and CTRP5 and calculation of CTRP1/CTRP5 levels are helpful for UAP screening and disease assessment.