鳗鱼肉粉中氨基甲苯咪唑基体标准物质的研制

Preparation of matrix reference material of amino-mebendazole in eel muscle powder

目的:建立鳗鱼肉粉中甲苯咪唑代谢物氨基甲苯咪唑基体标准物质的研制及定值方法。方法:通过药浴甲苯咪唑养殖鳗鱼的方式获得阳性样本,经冷冻干燥、真空包装和辐射灭菌等过程制得氨基甲苯咪唑基体标准物质,并考察其均匀性、稳定性、协同定值和不确定度。结果:该方法所制备的基体标样均匀性良好,能够适应低温、常温、长距离运输等环境,在-18℃下能够保存12个月以上,经8家资质检验机构的协同定值,氨基甲苯咪唑特性值为(35.3±6.4)μg/kg(k=2)。结论:该基体标准物质达到国家标准样品的技术要求,能够满足鳗鱼肉中氨基甲苯咪唑残留检测的质量控制和方法验证的需求。

Objective:A method for the preparation and determination of amino-mebendazole matrix reference material in eel muscle powder was established.Methods:A positive sample was obtained by culturing eels with mebendazole and was prepared into amino-mebendazole matrix reference material,which went through freeze-drying,vacuum packaging,and radiation sterilization in turn.And its homogeneity,stability,cooperative setting value,and uncertainty were examined.Results:This matrix reference material has good uniformity and can adapt to low temperatures,normal temperatures,long-distance transportation,and other environments.It can be stored for more than 12 months under-18℃.The characteristic value of amino-methylimidazole is(35.3±6.4)μg/kg(k=2)through the collaborative setting of 8 qualification inspection institutions.Conclusion:This matrix reference material meets the technical requirements of national standard samples and can meet the needs of quality control and method validation for amino-mebendazole residue detection in eel muscle.This study can provide a good reference for the development of food matrix reference materials.