AIM2炎性小体在早期非小细胞肺癌组织中的表达及其作用探讨

Expression and role of AIM2 inflammasome in early non-small cell lung cancer tissues

目的:观察黑素瘤缺乏因子2(AIM2)炎性小体在早期非小细胞肺癌(NSCLC)组织中的表达及其与临床病理特征的关系,探讨AIM2炎性小体在早期NSCLC发病中的作用及潜在机制。方法:收集57例早期NSCLC的癌组织及其癌旁组织石蜡标本,其中腺癌33例,鳞癌24例,采用免疫组织化学法检测肺癌及癌旁组织中AIM2炎性小体组分AIM2、凋亡相关斑点样蛋白(ASC)、caspase-1的蛋白表达,并检测其下游细胞因子白细胞介素(IL)-1β和IL-18的蛋白表达,分析其与临床病理特征的关系,探讨各蛋白表达之间的相关性。结果:NSCLC癌组织中炎性小体组分AIM2、ASC、caspase-1及IL-1β、IL-18的阳性表达率均明显高于癌旁组织(P<0.01)。AIM2、ASC、caspase-1、IL-1β、IL-18在腺癌中的阳性表达率均高于鳞癌(P<0.05~P<0.01);在低分化、伴有淋巴结转移癌组织中的阳性表达率均分别高于高中分化、无淋巴结转移的癌组织(P<0.05~P<0.01);不同性别、年龄、肿瘤直径癌组织中AIM2、ASC、caspase-1、IL-1β、IL-18的蛋白表达差异均无统计学意义(P>0.05)。在NSCLC癌组织中,AIM2的表达水平与ASC、caspase-1、IL-1β、IL-18的表达水平均呈正相关关系(P<0.05~P<0.01);ASC的表达水平与caspase-1、IL-1β、IL-18的表达水平均呈正相关关系(P<0.05~P<0.01);caspase-1的表达水平与IL-1β、IL-18的表达水平均呈正相关关系(P<0.01和P<0.05)。结论:早期NSCLC癌组织中AIM2炎性小体组分及IL-1β、IL-18表达均上调,且腺癌中AIM2炎性小体的表达高于鳞癌,其表达水平与肿瘤分化程度及有无淋巴结转移有关。

Objective:To observe the expression of absent in melanoma 2(AIM2)inflammasome in early non-small cell lung cancer(NSCLC)tissues and its relationship with clinicopathological features,and explore the role and potential mechanisms of AIM2 inflammasome in the pathogenesis of early NSCLC.Methods:The paraffin specimens of 57 early NSCLC tissues and their adjacent tissues were collected,including 33 cases of adenocarcinomas and 24 cases of squamous cell carcinoma.Immunohistochemistry assay was used to detect the protein expression of AIM2 inflammasome components AIM2,apoptosis-associated speck-like protein containing a CARD(ASC)and caspase-1,and the downstream cytokines interleukin(IL)-1βand IL-18,their relationship with clinicopathological features was analyzed,and the correlation between the expression of the above proteins was explored.Results:The positive expression rates of inflammasome components AIM2,ASC,caspase-1 and IL-1β,IL-18 in NSCLC tissues were significantly higher than those in adjacent tissues(P<0.01).The positive expression rates of AIM2,ASC,caspase-1,IL-1βand IL-18 in adenocarcinoma were higher than those in squamous cell carcinoma(P<0.05 to P<0.01),which in cancer tissues with low differentiation and lymph node metastasis were higher than those with high-middle differentiation and non-lymph node metastasis(P<0.05 to P<0.01),but the difference of which in cancer tissues with different gender,age and tumor size was not statistically significant(P>0.05).In NSCLC tissues,the expressionlevel of AIM2 was positively correlated with the expression level of ASC,caspase-1,IL-1βand IL-18(P<0.05 toP<0.01),theexpression level of ASC was positively correlated with the expression level of caspase-1,IL-1βand IL-18(P<0.05 toP<0.01),andthe expression level of caspase-1 was positively correlated with the expression level of IL-1βand IL-18(P<0.01 andP<0.05).Conclusions:The expression of AIM2 inflammasome components and IL-1β,IL-18 are all up-regulated in early NSCLC tissues,theexpression of AIM2 inflammasome in adenocarcinoma is higher than that in squamous cell carcinoma,and its expression level is relatedto the degree of tumor differentiation and lymph node metastasis.