基于SNP密度分布分析柔嫩艾美耳球虫地克珠利耐药虫株与敏感虫株的差异

Differences between Eimeria tenella Diclazuril-Resistant and Diclazuril-Sensitive Strains Analyzed by SNP Density Distribution

为明确地克珠利耐药虫株与敏感虫株在基因水平的差异,进而解析地克珠利耐药性产生的分子机制,本研究以实验室保存的2株柔嫩艾美耳球虫地克珠利耐药虫株和2株柔嫩艾美耳球虫地克珠利敏感虫株为研究对象,将4个虫株进行全基因组重测序(NGS),通过单核苷酸多态性(SNP)密度分布解析耐药株与敏感株之间的差异。结果显示,与敏感株相比,耐药株在第13号染色体0.1~1 Mb区间内的SNP比较富集。本研究结果为解析鸡球虫14条染色体上抗原变异位点和耐药性位点等的研究提供了科学依据。

In order to determine the genetic differences between Eimeria tenella diclazuril-resistant and diclazuril-sensitive strains,and to elucidate the molecular mechanism of diclazuril-resistant strains,this study sequenced two resistant strains and two sensitive strains preserved in the laboratory by next-generation sequencing(NGS)and then compared single nucleotide polymorphism(SNP)density distribution between drug-resistant and drug-sensitive strains.The results showed that relative to the drug-sensitive strains,the drug-resistant strains had a more frequent SNP distribution in the region of 0.1-1 Mb on chromosome 13.This study forms a solid basis for further analysis of antigenic variation sites and drug resistance sites on 14 chromosomes of chicken coccidia.