慢性牙周炎患者血清STAT6 mRNA、HMGB1、MMP-13水平分析及临床意义探讨

Analysis on serum STAT6 mRNA,HMGB1 and MMP-13 levels in patients with chronic periodontitis and the clinical significance

目的探讨慢性牙周炎患者血清信号转导和转录激活因子6(STAT6)mRNA、高迁移率族蛋白B1(HMGB1)、基质金属蛋白酶-13(MMP-13)水平与病情、炎症反应和牙周临床指标的相关性。方法将2019年2月至2022年2月该院收治的156例慢性牙周炎患者纳入研究作为牙周炎组,根据病情严重程度分为轻度组(80例)和中重度组(76例),另选取72例无口腔疾病的健康者作为对照组。检测受试者血清STAT6 mRNA、HMGB1、MMP-13、肿瘤坏死因子-α(TNF-α)、白细胞介素(IL)-1α、IL-1β、IL-6水平以及牙周炎患者出血指数(BI)、探诊深度(PD)和附着丧失(AL)。采用Pearson相关分析STAT6 mRNA、HMGB1、MMP-13和炎症因子、牙周临床指标之间相关性。采用受试者工作特征(ROC)曲线分析STAT6 mRNA、HMGB1、MMP-13诊断慢性牙周炎的价值。结果牙周炎组血清STAT6 mRNA、HMGB1、MMP-13、TNF-α、IL-1α、IL-1β、IL-6水平均高于对照组(P<0.05)。中重度组血清STAT6 mRNA、HMGB1、MMP-13、TNF-α、IL-1α、IL-1β、IL-6水平及PD、BI、AL均高于轻度组(P<0.05)。牙周炎组STAT6 mRNA、HMGB1、MMP-13与TNF-α、IL-1α、IL-1β、IL-6水平及PD、BI、AL均呈正相关(P<0.05)。联合检测STAT6 mRNA、HMGB1和MMP-13用于诊断慢性牙周炎的ROC曲线下面积大于STAT6 mRNA、HMGB1和MMP-13单项检测(P<0.05)。结论慢性牙周炎患者血清STAT6 mRNA、HMGB1、MMP-13水平增高与炎症反应和牙周组织破坏有关,STAT6 mRNA、HMGB1、MMP-13可作为慢性牙周炎诊断的参考指标。

Objective To investigate the correlation between serum signal transducer and activator of transcription 6(STAT6)mRNA,high mobility group protein B1(HMGB1),and matrix metalloproteinase-13(MMP-13)levels and disease,inflammatory response,and periodontal clinical indicators in patients with chronic periodontitis.Methods A total of 156 patients with chronic periodontitis admitted to the hospital from February 2019 to February 2022 were enrolled in the study as the periodontitis group,and were divided into mild(80 cases)and moderate-to-severe(76 cases)group according to the severity of the disease,while 72 healthy individuals without oral disease were enrolled as the control group.Serum STAT6 mRNA,HMGB1,MMP-13,tumor necrosis factor-α(TNF-α),interleukin(IL)-1α,IL-1β,IL-6 levels as well as bleeding index(BI),probing depth(PD)and attachment loss(AL)in patients with periodontitis were measured.Pearson correlation analysis was used to analyze the correlation between STAT6 mRNA,HMGB1,MMP-13 and inflammatory factors and periodontal clinical indicators.The values of STAT6 mRNA,HMGB1,and MMP-13 for the diagnosis of chronic periodontitis were analyzed using the subject operating characteristic(ROC)curve.Results Serum STAT6 mRNA,HMGB1,MMP-13,TNF-α,IL-1α,IL-1β,and IL-6 levels were higher in the periodontitis group than those in the control group(P<0.05).Serum STAT6 mRNA,HMGB1,MMP-13,TNF-α,IL-1α,IL-1β,IL-6 levels and PD,BI,AL were higher in the moderate-to-severe group than those in the mild group(P<0.05).STAT6 mRNA,HMGB1,and MMP-13 were positively correlated with TNF-α,IL-1α,IL-1β,IL-6 levels and PD,BI,and AL in the periodontitis group(P<0.05).The area under the ROC curve of combined detection of STAT6 mRNA,HMGB1 and MMP-13 for the diagnosis of chronic periodontitis was greater than that of STAT6 mRNA,HMGB1 and MMP-13 used alone(P<0.05).Conclusion The increased levels of STAT6 mRNA,HMGB1 and MMP-13 in serum of patients with chronic periodontitis are related to inflammatory response and periodontal tissue destruction.STAT6 mRNA,HMGB1 and MMP-13 can be used as reference indicators for the diagnosis of chronic periodontitis.