G-四链体荧光生物传感器铅快速分析技术研究进展

Research progress of G-quadruplex fluorescent biosensor for rapid analysis of lead

铅是原子量最大的非放射性剧毒重金属,在环境中很难被其他物质降解消除,能长时间保持毒性,而产生持续性的健康危害。为了防范重金属铅污染,避免污染事件的屡次发生,做好环境中的重金属铅含量监测意义重大。核酸适配体构建的生物传感器作一种优良重金属铅离子检测方法成为研究的热点。本文介绍了G-四链体型核酸适配体概念及其与铅离子识别机制,综述了近10年来基于G-四链体荧光生物传感器分析技术在铅离子检测中的研究、创新和存在问题,并展望了其发展方向。

Lead is the non-radioactive and highly toxic heavy metal with the largest atomic weight.It is difficult to be degraded and eliminated by other substances in the environment.It can remain toxic for a long time and cause continuous health hazards.In order to prevent heavy metal lead pollution and avoid the repeated occurrence of pollution incidents,it is of great significance to do a good job in monitoring the heavy metal lead content in the environment.The biosensor constructed by nucleic acid aptamer as an excellent method for the detection of heavy metal lead ion has become a research hotspot.The paper introduces the concept of G-quadruplex nucleic acid aptamer and its recognition mechanism with lead ions,summarizes the research,innovation and existing problems in the detection of lead ions based on G-quadruplex fluorescent biosensor analysis technology in recent 10 years,and looks forward to its development direction.