云南省暗娼人群生殖道沙眼衣原体基因型分析

Analysis of Chlamydia Trachomatis Genotype among Female Sex Workers in Yunnan Province

目的 了解云南省暗娼人群中生殖道沙眼衣原体(chlamydia trachomatis,CT)的亚型分布情况及流行病学特征。方法 2020年结合艾滋病哨点监测开展生殖道沙眼衣原体调查,收集生殖道沙眼衣原体核酸阳性样本374例,使用巢式PCR扩增CT的OmpA基因区并进行分析。结果 在173个成功获得序列的样本中,共检测出7种基因型,其中以E型、F型、J型和D型为主要基因型,分别占24.9%(43/173)、21.4%(37/173)、19.1%(33/173)、17.9%(31/173),其他包括G型(9.3%,16/173)、K型(4.6%,8/173)和H型(2.9%,5/173)。不同型别之间在调查地区、户籍、是否为边境州(市)、年龄、民族、文化程度、婚姻状况、样本来源和场所档次等特征上差异无统计学意义(P> 0.05)。结论 云南省暗娼人群中存在多种的CT亚型,其分布在主要的人口学特征上无差异,可为进一步控制CT的传播提供指导,也可为将来治疗和疫苗研究提供基础信息。

Objective To investigate the serovar distribution and epidemiological characteristics of Chlamydia trachomatis( CT) in female sex workers( FSWs) from Yunnan Province. Methods A survey of Chlamydia trachomatis in the genital tract was conducted in 2020 in conjunction with HIV sentinel surveillance,and374 nucleic acid-positive samples of Chlamydia trachomatis in the genital tract were collected. The OmpA gene region of CT was amplified by nested polymerase chain reaction(PCR) and analyzed. Results Seven genotypes were detected in 173 samples successfully genotyped,among which genotype E(24.9%,43/173),genotype F(21.4%,37/173),genotype J(19.1%,33/173),genotype D(17.9%,31/173) were the main genotypes,and the others included genotype G(9.3%,16/173),genotype K(4.6%,8/173)and genotype H(2.9%,5/173). No statistically significant differences were found among the different genotypes in characteristics such as survey area,household registration,whether it was a border state(city),age,ethnicity,education level,marital status, sample source,and place class. Conclusion Multiple subtypes of CT exist in FSWs in Yunnan Province,and their distribution does not differ in terms of major demographic characteristics. This study may provide guidance for further control of CT transmission and provide basic information for future treatment and vaccine studies.