摘要
目的 探讨重楼皂苷I(PPI)对人乳腺癌敏感及耐药细胞膜通透性和流动性的影响。方法 将MCF-7、MCF-7/ADM细胞分别分为空白组(0μmol·L^(-1)PPI)和低、中、高、超高浓度实验组(0.3,1.0,3.0和10.0μmol·L^(-1)PPI)。用改良噻唑蓝法检测细胞的增殖抑制率,用锥虫蓝染色法、乳酸脱氢酶(LDH)释放法检测细胞膜通透性,用激光共聚焦显微镜检测细胞的荧光漂白恢复率。结果 对于MCF-7细胞,高浓度实验组和空白组3 h的抑制率分别为(14.79±9.50)%和(0.00±0.00)%,48 h的锥虫蓝阳染率分别为(91.28±3.51)%和(0.00±0.00)%,24 h的LDH释放率分别为(68.64±5.86)%和(6.48±0.50)%,差异均有统计学意义(均P<0.05)。对于MCF-7/ADM细胞,高浓度实验组和空白组3 h的抑制率分别为(24.87±8.00)%和(0.00±0.00)%,48 h的锥虫蓝阳染率分别为(92.61±4.23)%和(0.00±0.00)%,24 h的LDH释放率分别为(67.58±8.20)%和(5.00±1.73)%,差异均有统计学意义(P<0.01,P<0.001)。MCF-7/ADM细胞的超高浓度实验组和空白组1 h的荧光漂白恢复率分别为(26.80±4.77)%和(43.52±3.35)%,差异有统计学意义(P<0.01)。结论 PPI可增加人乳腺癌敏感及耐药细胞的膜通透性,降低耐药细胞的膜流动性。
Objective To investigate the effect of Polyphyllin I(PPI) on membrane permeability and fluidity of MCF-7 and MCF-7/ADM cells. Methods MCF-7 and MCF-7/ADM cells were divided into blank(0 μmol·L^(-1)PPI) group and experimental-L,-M,-H,-UH(0.3, 1.0, 3.0, 10.0 μmol·L^(-1)PPI) groups. The proliferation inhibition rate was detected by modified methyl thiazolyl tetrazolium method. The cell membrane permeability was detected by trypan blue staining and lactate dehydrogenase(LDH) release assay. The fluorescence bleaching recovery rate of cells was detected by laser confocal microscope. Results For MCF-7 cells, in experimental-H group and blank group, the inhibition rates at 3 h were(14.79±9.50)% and(0.00±0.00)%;trypan blue positive staining rates at 48 h were(91.28±3.51)% and(0.00±0.00)%;LDH release rates at 24 h were(68.64±5.86)% and(6.48±0.50)%;the differences were statistically significant(all P<0.05). For MCF-7/ADM cells, in experimental-H group and blank group,the inhibition rates at 3 h were( 24. 87 ± 8. 00) % and( 0. 00 ± 0. 00) %;trypan blue positive staining rates at 48 h were( 92. 61 ± 4. 23) % and( 0. 00 ± 0. 00) %;LDH release rates at 24 h were( 67. 58 ± 8. 20) %and( 5. 00 ± 1. 73) %;the differences were statistically significant( P < 0. 01,P < 0. 001). The fluorescence bleaching recovery rates of MCF-7/ADM cell at 1 h in experimental-UH group and blank group were( 26. 80 ± 4. 77) % and( 43. 52 ±3. 35) %;the difference was statistically significant( P < 0. 01). Conclusion PPI can increase the membrane permeability of MCF-7 and MCF-7/ADM cells,and decrease the membrane fluidity of MCF-7/ADM cells.
作者
朱翔
柴冬亚
张六一
徐杨安泰
张园
周轶平
ZHU Xiang;CHAI Dong-ya;ZHANG Liu-yi;XUYANG An-tai;ZHANG Yuan;ZHOU Yi-ping(School of Pharmaceutical Sciences&Yunnan Key Laboratory of Pharmacology for Natural Products,Kunming Medical University,Kunming 650500,Yunnan Province,China;Department of Pharmacy,The Third People’s Hospital of Kunming,Kunming 650041,Yunnan Province,China;Department of Pharmacy Intravebous Admixture Services,The People’s Hospital of Chuxiong Yi Autonomous Prefecture,Chuxiong 675000,Yunnan Province,China)
出处
《中国临床药理学杂志》
CAS
CSCD
北大核心
2023年第2期196-200,共5页
The Chinese Journal of Clinical Pharmacology
基金
国家自然科学基金地区基金资助项目(81960739)
云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目(2019FE001-193)
云南省科学技术院重大科技专项生物资源数字化开发应用基金资助项目(202002AA100007)。
关键词
重楼皂苷I
人乳腺癌细胞
细胞膜
通透性
流动性
Polyphyllin I
human breast cancer cell
cell membrane
permeability
fluidity