重楼皂苷I对人乳腺癌细胞膜通透性和流动性的影响

Effect of Polyphyllin I on membrane permeability and fluidity of human breast cancer cells

目的 探讨重楼皂苷I(PPI)对人乳腺癌敏感及耐药细胞膜通透性和流动性的影响。方法 将MCF-7、MCF-7/ADM细胞分别分为空白组(0μmol·L^(-1)PPI)和低、中、高、超高浓度实验组(0.3,1.0,3.0和10.0μmol·L^(-1)PPI)。用改良噻唑蓝法检测细胞的增殖抑制率,用锥虫蓝染色法、乳酸脱氢酶(LDH)释放法检测细胞膜通透性,用激光共聚焦显微镜检测细胞的荧光漂白恢复率。结果 对于MCF-7细胞,高浓度实验组和空白组3 h的抑制率分别为(14.79±9.50)%和(0.00±0.00)%,48 h的锥虫蓝阳染率分别为(91.28±3.51)%和(0.00±0.00)%,24 h的LDH释放率分别为(68.64±5.86)%和(6.48±0.50)%,差异均有统计学意义(均P<0.05)。对于MCF-7/ADM细胞,高浓度实验组和空白组3 h的抑制率分别为(24.87±8.00)%和(0.00±0.00)%,48 h的锥虫蓝阳染率分别为(92.61±4.23)%和(0.00±0.00)%,24 h的LDH释放率分别为(67.58±8.20)%和(5.00±1.73)%,差异均有统计学意义(P<0.01,P<0.001)。MCF-7/ADM细胞的超高浓度实验组和空白组1 h的荧光漂白恢复率分别为(26.80±4.77)%和(43.52±3.35)%,差异有统计学意义(P<0.01)。结论 PPI可增加人乳腺癌敏感及耐药细胞的膜通透性,降低耐药细胞的膜流动性。

Objective To investigate the effect of Polyphyllin I(PPI) on membrane permeability and fluidity of MCF-7 and MCF-7/ADM cells. Methods MCF-7 and MCF-7/ADM cells were divided into blank(0 μmol·L^(-1)PPI) group and experimental-L,-M,-H,-UH(0.3, 1.0, 3.0, 10.0 μmol·L^(-1)PPI) groups. The proliferation inhibition rate was detected by modified methyl thiazolyl tetrazolium method. The cell membrane permeability was detected by trypan blue staining and lactate dehydrogenase(LDH) release assay. The fluorescence bleaching recovery rate of cells was detected by laser confocal microscope. Results For MCF-7 cells, in experimental-H group and blank group, the inhibition rates at 3 h were(14.79±9.50)% and(0.00±0.00)%;trypan blue positive staining rates at 48 h were(91.28±3.51)% and(0.00±0.00)%;LDH release rates at 24 h were(68.64±5.86)% and(6.48±0.50)%;the differences were statistically significant(all P<0.05). For MCF-7/ADM cells, in experimental-H group and blank group,the inhibition rates at 3 h were( 24. 87 ± 8. 00) % and( 0. 00 ± 0. 00) %;trypan blue positive staining rates at 48 h were( 92. 61 ± 4. 23) % and( 0. 00 ± 0. 00) %;LDH release rates at 24 h were( 67. 58 ± 8. 20) %and( 5. 00 ± 1. 73) %;the differences were statistically significant( P < 0. 01,P < 0. 001). The fluorescence bleaching recovery rates of MCF-7/ADM cell at 1 h in experimental-UH group and blank group were( 26. 80 ± 4. 77) % and( 43. 52 ±3. 35) %;the difference was statistically significant( P < 0. 01). Conclusion PPI can increase the membrane permeability of MCF-7 and MCF-7/ADM cells,and decrease the membrane fluidity of MCF-7/ADM cells.