2,4-二硝基氯苯诱导BALB/c小鼠特应性皮炎模型的建立

Establishment of Atopic Dermatitis Model Induced by DNCB in BALB/C mice

目的利用2,4-二硝基氯苯(DNCB)诱导BALB/c小鼠建立特应性皮炎(AD)模型,探讨最适条件。方法给予BALB/c小鼠背部皮肤不同药物浓度以及不同的造模时间致敏及激发,在末次给药后,评估小鼠背部皮肤状态。取背部皮肤组织做组织病理学检测,分离血清检测免疫球蛋白E(IgE)水平,制备皮肤匀浆液检测细胞因子白细胞介素-4(IL-4)、白细胞介素-5(IL-5)及白细胞介素-13(IL-13)分泌水平。结果4组造模模型组动物中,D组小鼠0.5%DNCB致敏3次,1%DNCB激发6次,其皮肤皮炎评分最高,病理结果显示皮肤结构不完整,棘层明显增厚,细胞内水肿明显,大量炎性细胞浸润,血清IgE水平升高,皮肤组织细胞因子明显升高,相比于其他模型组其各种检测指标更接近特应性皮炎皮肤改变。结论早期低剂量致敏,后期高剂量激发可成功建立小鼠AD模型,这为进行AD的相关科学研究提供了一定的实验依据。

Objective To establish atopic dermatitis(AD) model in BALB/c mice induced by 2,4-dinitrochlorobenzene(DNCB),and to explore the optimal conditions,so as to provide theoretical basis for the establishment of animal model in the research of AD.Method BALB/C mice were sensitized and stimulated with different drug concentrations and different modeling time.After the last administration,the severity of dermatitis in the dorsal skin of DNCB-induced AD mice was evaluated based on symptoms,and hematoxylin and eosin(H&E) and toluidine blue(TB) staining were carried out for further analysis.Serum samples were collected for immunoglobulin E(IgE) detection,and the skin homogenates were prepared to detect the levels of interleukin-4(IL-4),interleukin-5(IL-5) and interleukin-13(IL-13).Result Mice in group D were sensitized by 0.5% DNCB for 3 times and stimulated by 1% DNCB for 6 times.The skin severity score was the highest in group D.The pathological result showed that the skin structure was incomplete,the Spinous layer was obviously thickened,the intracellular edema was obvious,and a large number of inflammatory cells were infiltrated,the levels of serum IgE and cytokines in skin tissue were significantly higher than other groups.Conclusion Low-dose sensitization at early stage and high-dose stimulation at late stage can establish mouse AD model successfully,which lays a foundation for further study on AD.