假性蛋白激酶3及微小RNA-1827在结直肠癌中的表达及意义

Expression and significance of Tribbles 3 and microRNA-1827 in colorectal cancer

目的探讨假性蛋白激酶3(Trib3)、微小RNA-1827(miR-1827)在结直肠息肉和结直肠癌中的表达及意义。方法选取97例结直肠癌患者癌组织作为结直肠癌组,选取同期86例结直肠息肉患者息肉组织作为息肉组,选取同期行结直肠镜检查的92例健康体检者正常结直肠黏膜组织作为对照组。采用免疫组织化学法检测不同组织中Trib3表达水平,采用实时荧光定量PCR(qRT-PCR)法检测不同组织中miR-1827表达水平;分析Trib3、miR-1827表达水平与结直肠息肉和结直肠癌临床病理特征的关系;分析结直肠息肉组织及结直肠癌组织中Trib3与miR-1827的相关性;采用多因素COX回归分析探讨影响结直肠息肉和结直肠癌患者预后的因素。结果结直肠癌组Trib3高表达比率高于息肉组和对照组,miR-1827水平低于息肉组和对照组;息肉组Trib3高表达比率高于对照组,miR-1827水平低于对照组;上述组间差异均有统计学意义(P<0.01)。Trib3、miR-1827表达水平与结直肠息肉患者息肉直径、组织病理学分型、息肉数目有相关性(P<0.01)。Trib3、miR-1827表达水平与结直肠癌患者肿瘤直径、临床分期、淋巴结转移、分化程度有相关性(P<0.05)。Trib3、miR-1827在结直肠息肉组织及结直肠癌组织中均呈负相关(r=-0.349、-0.397,P<0.05)。Trib3是结直肠息肉患者治疗无效的独立危险因素(P<0.05),miR-1827是结直肠息肉患者治疗无效的保护因素(P<0.05)。Trib3、临床分期是结直肠癌患者死亡的独立危险因素(P<0.05),miR-1827是结直肠癌患者死亡的保护因素(P<0.05)。结论Trib3、miR-1827表达水平在结直肠黏膜组织癌变进程中分别上调和下调,检测二者表达变化可监测结直肠息肉恶变过程,进而早期预防结直肠癌病变。

Objective To investigate the expressions and significance of Tribbles 3(Trib3)and microRNA-1827(miR-1827)in colorectal polyps and colorectal cancer.Methods The cancer tissues of 97 patients with colorectal cancer were selected as colorectal cancer group,the polyp tissues of 86 patients with colorectal polyps in the same period were selected as polyp group,and the normal colorectal mucosal tissues of 92 healthy people with colorectal endoscopy in the same period were selected as control group.The expressions of Trib3 in different tissues were detected by immunohistochemistry,and real-time fluorescent quantitative PCR(qRT-PCR)method was used to detect the expression level of miR-1827 in different tissues;the relationships of the Trib3 and miR-1827 levels with the clinicopathological characteristics of colorectal polyps and colorectal cancer were analyzed;the correlations between Trib3 and miR-1827 in colorectal polyps and colorectal cancer tissues were analyzed;the multivariate COX regression analysis was used to explore the prognostic factors in patients with colorectal polyps or colorectal cancer.Results The high expression rate of Trib3 in the colorectal cancer group was higher than that in the polyp group and the control group,while the level of miR-1827 was lower than that in the polyp group and the control group;the high expression rate of Trib3 in the polyp group was higher than that in the control group,while the level of miR-1827 was lower than that in the control group;the between-group differences mentioned above were statistically significant(P<0.01).The expression levels of Trib3 and miR-1827 were correlated with the polyp diameter,histopathological types and the number of polyps in patients with colorectal polyps(P<0.01).The expression levels of Trib3 and miR-1827 were correlated with the tumor diameter,clinical staging,lymph node metastasis and degree of differentiation in patients with colorectal cancer(P<0.05).Trib3 was negatively correlated with miR-1827 in both colorectal polyps and colorectal cancer tissues(r=-0.349,-0.397,P<0.05).Trib3 was an independent risk factor for ineffective treatment of colorectal polyps(P<0.05),and miR-1827 was a protective factor for ineffective treatment of colorectal polyps(P<0.05).Trib3 and clinical staging were the independent risk factors for death in patients with colorectal cancer(P<0.05),and miR-1827 was a protective factor for death in patients with colorectal cancer(P<0.05).Conclusion The expression levels of Trib3 and miR-1827are up-regulated and down-regulated respectively in the carcinogenesis process of colorectal mucosal tissues,the detection on expression changes of the two indexes can monitor the malignant process of colorectal polyps,and carry out the early prevention of colorectal cancer.