抵抗素基因多态性与结直肠癌易感性的相关性

Correlation between resistin gene polymorphism and colorectal cancer susceptibility

目的探讨抵抗素基因多态性与结直肠癌易感性的相关性。方法选取56例结直肠癌患者为研究对象(病例组),选取同期52例结直肠腺瘤患者为对照组,选取同期60例健康体检者作为健康组。提取3组血清基因组DNA,采用聚合酶链反应(PCR)法扩增抵抗素基因rs1862513片段并进行基因测序,探究其基因多态性;采用酶联免疫吸附(ELISA)法检测血清抵抗素、脂联素和瘦素水平;比较3组抵抗素基因多态性位点rs1862513基因型分布;采用Logistic回归分析法分析影响结直肠腺癌发生的因素。结果抵抗素基因在rs1862513位点上有GG型、GC型、CC型共3种基因型,健康组中GG型基因型最多,CC型基因型最少;对照组GC型基因型最多,CC型基因型最少;病例组CC型基因型最多,GG型基因型最少。病例组、对照组及健康组各基因型符合Hardy-Weinberg平衡定律(P>0.05)。病例组抵抗素水平低于健康组和对照组,脂联素、瘦素水平高于健康组和对照组,差异有统计学意义(P<0.05);对照组抵抗素水平低于健康组,脂联素、瘦素水平高于健康组,差异有统计学意义(P<0.05)。CC型结直肠癌患者血清抵抗素水平低于GG型和GC型,脂联素、瘦素水平高于GG型和GC型,差异有统计学意义(P<0.05);GC型结直肠癌患者血清抵抗素水平低于GG型,脂联素、瘦素水平高于GG型,差异有统计学意义(P<0.05)。基因型CC型是结直肠癌的独立危险因素(P<0.05)。结论抵抗素CC型基因型与结直肠癌易感性有一定相关性,或可成为结直肠癌患者诊断和治疗潜在的基因标志物。

Objective To investigate the relationship between resistin gene polymorphism and colorectal cancer susceptibility.Methods A total of 56 patients with colorectal cancer were selected as study subjects(case group).Fifty-two patients with colorectal adenoma were selected as control group.During the same period,60 healthy subjects were selected as healthy group.The serum genomic DNA of the three groups was extracted,rs1862513 fragment of resistin gene was amplified by polymerase chain reaction(PCR)and was sequenced,and its gene polymorphism was explored;the levels of resistin,adiponectin and leptin were detected by enzyme-linked immunosorbent assay(ELISA);the genotype distribution of rs1862513 was compared among the three groups;Logistic regression analysis was used to analyze the factors affecting the occurrence of colorectal adenocarcinoma.Results There were three genotypes of resistin gene at rs1862513 locus:GG,GC and CC genotypes.In the healthy group,GG genotype was the most,CC genotype was the least;in the control group,GC genotype was the most,CC genotype was the least;in the case group,CC genotype was the most,GG genotype was the least.The each genotype in case group,control group and healthy group were in accordance with Hardy-Weinberg equilibrium law(P>0.05).The level of resistin in the case group was significantly lower than that in the healthy group and the control group,and the level of adiponectin and leptin was significantly higher than those in the healthy group and the control group(P<0.05);the level of resistin in the control group was significantly lower than that in the healthy group,and the levels of adiponectin and leptin were significantly higher than those in the healthy group(P<0.05).The level of serum resistin of CC type colorectal cancer patients was significantly lower than that of GG type and GC type,and the levels of adiponectin and leptin were significantly higher than that of GG type and GC type(P<0.05);the level of serum resistin of GC type colorectal cancer patients was significantly lower than that of GG type,and the levels of adiponectin and leptin were significantly higher than that of GG type(P<0.05).The CC genotype was an independent risk factor for colorectal cancer(P<0.05).Conclusion Resistin CC genotype is associated with colorectal cancer susceptibility,and may provide potential genetic markers for diagnosis and treatment of colorectal cancer patients.