丹寿汤对抗心磷脂抗体致妊娠丢失孕鼠子宫螺旋动脉重铸障碍的影响

Effect of Danshou Decoction on Uterine Spiral Artery Recasting Disturbance in Rats with Pregnancy Loss Caused by Anti-cardiolipin Antibody

目的 探讨丹寿汤对抗心磷脂抗体(ACA)致妊娠丢失孕鼠子宫螺旋动脉重铸障碍的影响。方法 以人ACA标准蛋白液为诱导剂制备ACA阳性孕鼠模型。实验随机均分为空白组、模型组、阳性药组、丹寿汤组。自受孕日起,空白组和模型组给予蒸馏水灌胃,阳性药组给予低分子肝素钙注射液[558 IU/(kg·d)]皮下注射,丹寿汤组给予丹寿汤药液[11.2 g/(kg·d)]灌胃,持续给药15d后处死取材。计算胚胎吸收率,HE染色法观察胎盘组织病理形态,Case Viewer图像分析测量胎盘血管管腔直径与血管密度,ELISA法检测血清白介素-6(IL-6)与血管内皮生长因子(VEGF)水平。结果 与模型组相比,阳性药组和丹寿汤组孕鼠的胚胎和胎盘质量显著增加,胚胎吸收率显著降低,胎盘血管管腔直径和血管密度显著扩增,血清IL-6水平显著降低,且血清VEGF水平显著升高(均P<0.05)。结论 丹寿汤对ACA致妊娠丢失孕鼠有明确保护作用,其机制可能与通过抗炎和促进血管新生改善子宫螺旋动脉重铸障碍有关。

Objective To investigate the effect of Danshou Decoction on uterine spiral artery(SPA) recasting disorder in rats with pregnancy loss induced by anti-cardiolipin antibody(ACA). Methods ACA standard protein solution of human was used as inducer to establish ACA positive pregnant rat model, which were randomly divided into control, model, positive drug and Danshou decoction groups. From the day of conception, the rats in control and model groups were given distilled water, the rats in positive drug group were injected subcutaneously low molecular weight heparin [558 IU/(kg·d)], and the rats in Danshou decoction group were given Danshou decoction liquid [11.2 g/(kg·d)] by gavage. All rats were killed after continuous administration for 15 days. The embryo resorption rate was calculated, the pathological morphology of placental tissue was observed by HE staining, the diameter and density of placental vascular lumen were measured by CaseViewer image analysis, and the contents of serum interleukin-6(IL-6) and vascular endothelial growth factor(VEGF) were measured by ELISA method. Results Compared with the model group, the quality of embryo and placenta significantly increased, the absorption rate of embryo significantly decreased, the diameter and density of placental vascular lumen significantly improved, the content of serum IL-6 significantly decreased, and the content of serum VEGF significantly increased in the positive drug and Danshou decoction groups(all P< 0.05). Conclusion Danshou decoction has a definite effect on rats with pregnancy loss caused by ACA, which may improve the disturbance of uterine SPA recasting by anti-inflammation and promoting angiogenesis.