改良QuEChERS-气相色谱质谱联用法测定金银花中农药残留

A modified QuEChERS-gas chromatography-mass spectrometry method for the determination of pesticide residues in Lonicera japonicae Flos

建立了一种以多壁碳纳米管(MWCNTs)为吸附剂的QuEChERS-气质联用法(GC-MS/MS)测定金银花中72种农药残留的分析方法。样品采用美国官方农业化学家协会(AOAC)提取包提取,MWCNTs改良的净化管净化,动态多反应监测模式(dMRM)分析,基质匹配标准曲线外标法定量。结果表明,72种农药在各自线性范围内呈良好的线性关系,相关系数均大于0.9936,检出限在0.001~0.178μg/kg之间,定量限在0.002~0.593μg/kg之间,4个添加水平的回收率为60.1%~118.0%,相对标准偏差(RSD)为2.2%~17%。

A modified QuEChERS-gas chromatography-mass spectrometry method using multi-walled carbon nanotubes(MWCNTs) as adsorbent was established for the determination of 72 pesticide residues in Lonicera japonicae Flos. The sample was extracted with Association of Official Agricultural Chemists(AOAC) extracting package, purified with MWCNTs-modified purification tube, analyzed with dynamic multiple reaction monitoring mode, and quantified with external standard method of matrix-matched standard curve. The 72 pesticides showed good linearities in the corresponding concentration ranges with correlation coefficients greater than 0.9936. The limits of detection were 0.001-0.178 μg/kg, and the limits of quantitation were 0.002-0.593 μg/kg. The average recoveries for four spiked levels ranged from 60.1% to 118.0%, with the relative standard deviations of 2.2%-17%.