益肾化瘀汤对糖尿病肾病大鼠肾功能及外周血腺苷脱氨酶表达的影响

Effect of Yishen Huayu Decoction on Renal Function and Expression of Peripheral Dlood Adenosine Deaminase in the Treatment of Diabetic Kidney Disease

目的:研究益肾化瘀汤对糖尿病肾病(diabetic kidney disease,DKD)大鼠肾功能及外周血腺苷脱氨酶(adenosine deaminase,ADA)表达的影响。方法:将30只大鼠随机分为空白组、模型组和治疗组,每组10只。模型组和治疗组均建立DKD模型,治疗组给予益肾化瘀汤治疗30 d。分别于给药后的第1 d、第10 d、第20 d和第30 d检测各组大鼠的血糖水平、肾功能指标和血清ADA的表达水平,HE染色观察肾组织病理学变化。结果:模型组大鼠出现多饮多尿体重减轻的糖尿病症状,治疗组在给予益肾化瘀汤后上述症状均有所好转。模型组和治疗组的空腹血糖值(fasting blood glucose,FBG)水平均显著高于空白组(P<0.05),自第10 d开始治疗组大鼠FBG水平显著低于模型组(P<0.05)。模型组和治疗组大鼠血清肌酐(serum creatinine,SCr)和血尿素氮(blood urea nitrogen,BUN)的表达水平显著高于空白组(P<0.05),自第10 d开始治疗组大鼠的Scr和BUN显著低于模型组(P<0.05)。模型组和治疗组大鼠血清ADA的表达水平显著高于空白组(P<0.05),自第10 d开始治疗组大鼠ADA的表达显著低于模型组(P<0.05)。结论:益肾化瘀汤可显著改善DKD大鼠的血糖水平、肾功能及血清ADA表达水平,具有一定的临床应用价值。

Objective:To study the effect of Yishen Huayu decoction on renal function and expression of peripheral blood adenosine deaminase(ADA)in the treatment of diabetic kidney disease(DKD).Methods:30 rats were randomly divided into blank group,model group and treated group,with 10 rats in each group.The model group and treated group were established DKD model,the treated group were administrated with Yishen Huayu decoction for 30 days.The fasting blood glucose,renal function indexes and expression level of serum ADA of each group were detected at 1 d,10 d,20 d and 30 d after drug delivered,pathological changes of renal tissue were observed by HE staining.Results:Diabetic symptoms of polydipsia,polyuria,weight loss were appeared with model group and treated group,and the treated group was improved after drug delivered.The FBG of model group and treated group were higher than blank group(P<0.05),and the treated group was lower than model group from 10 d after drug delivered(P<0.05).The serum creatinine(SCr)and blood urea nitrogen(BUN)of model group and treated group were higher than blank group(P<0.05),and the treated group was lower than model group from 10 d after drug delivered(P<0.05).The expression level of ADA of model group and treated group were higher than blank group(P<0.05),and the treated group was lower than model group from 10 d after drug delivered(P<0.05).Conclusion:Yishen Huayu decoction could significantly alleviate the blood glucose level,renal function and expression of ADA of DKD rats,which had certain clinical application value.