荧光光谱法研究单壁碳纳米管与牛血红蛋白的相互作用

Fluorescence Spectroscopic Investigation of Interaction of Single-Walled Carbon Nanotubes with Bovine Hemoglobin

选取了单壁碳纳米管(SWCNTs)及两种分离后的单一手性单壁碳纳米管(6,5)-SWCNTs、(8,3)-SWCNTs分别与牛血红蛋白(BHb)结合,通过荧光光谱法分析SWCNTs与BHb的相互作用。结果表明,SWCNTs对BHb的荧光猝灭为动态猝灭与静态猝灭结合的机制,(6,5)-SWCNTs和(8,3)-SWCNTs对BHb的荧光猝灭属于静态猝灭的机制;298 K下BHb与3种SWCNTs的结合常数大小顺序如下:SWCNTs>(6,5)-SWCNTs>(8,3)-SWCNTs;范德华力、氢键和疏水作用是相互作用中的主要作用力。

Single-walled carbon nanotubes(SWCNTs) can combine with proteins in organisms, creating potential biosafety risks. In this paper, SWCNTs and two separated single-chiral single-walled carbon nanotubes((6,5)-SWCNTs,(8,3)-SWCNTs) were combined with bovine hemoglobin(BHb), respectively, and interaction between SWCNTs and BHb was analyzed by fluorescence spectroscopy. The results showed that the fluorescence quenching of BHb by SWCNTs was stemmed from the combination of dynamic quenching and static quenching. The fluorescence quenching of BHb by single chiral(6,5)-SWCNTs and(8,3)-SWCNTs was static quenching. At 298 K, the order of the binding constants of BHb with different SWCNTs were as follows: SWCNTs >(6,5)-SWCNTs >(8,3)-SWCNTs. Van der Waals force, hydrogen bond and hydrophobic interaction were the main forces in the interaction.