基于转录组测序技术研究高原低氧对小鼠脾脏组织的影响

Study on effect of plateau hypoxia on mice spleen using transcriptome sequencing

目的:利用转录组学测序技术探究免疫系统在高原低氧胁迫适应过程中相关基因的表达及响应的分子机制。方法:本研究在高、低海拔环境分别饲养C57BL/6小鼠30 d,取脾脏组织利用RNA-Seq进行转录组测序,将得到的差异基因(DEGs)进行GO和KEGG富集分析,并通过荧光定量PCR验证测序数据的准确性。结果:与平原常氧组相比,共富集到4218个DEGs(P<0.05)。其中,ANXA1、S100A8、S100A9和HSPB1等基因显著富集;GO结果表明DEGs主要富集于B细胞激活、免疫球蛋白复合体和抗原结合分类,且JAK-STAT及NOD样受体信号通路为显著富集通路。结论:免疫系统响应高原低氧胁迫的转录调控分子可能致使机体内部免疫调节和炎症反应失衡,为相关高原病的病因学探究提供了新的理论依据。

Objective:The transcriptome sequencing technology was used to explore the molecular mechanism of the expression and response of the relevant genes in the immune system adaptation to high altitude hypoxia stress.Methods:In this study,C57BL/6 mice were raised at high and low altitudes respectively for 30 days.Spleen tissues were taken for transcriptional sequencing using RNA Seq.GO and KEGG enrichment analysis were performed on the DEGs obtained,and the accuracy of sequencing data was verified by fluorescence quantitative PCR.Results:Compared with plain normoxia group,4218 DEGs were enriched(P<0.05).Among them,ANXA1,S100A8,S100A9 and HSPB1 were significantly enriched;GO results showed that the DEGs were mainly enriched in B cell activation,immunoglobulin complex and antigen binding classification,JAK-STAT and NOD-LIKE receptor signaling pathways were significantly enriched.Conclusion:Transcription regulatory molecules of immune system in response to high altitude hypoxia stress may lead to the imbalance of immune regulation and inflammatory response.This study provides a new theoretical basis for the etiology of related high altitude diseases.