西南地区男/女儿童血清抗米勒管激素水平医学参考值范围的建立

Establishment of medical reference range for serum anti-Mullerian hormone levels in male/female children in Southwest China

目的建立西南地区健康体检儿童不同年龄段抗米勒管激素(AMH)的正常参考值区间。方法选取2021年4月至2022年1月该院健康体检儿童(0~16岁)为研究对象,采用化学发光免疫分析法测定血清AMH水平。经筛查最终纳入269例合格儿童的血清AMH值。采用统计学软件SPSS 19.0分析数据,正态分布的计量资料以95%置信区间(95%CI)表示参考范围,非正态分布的计量资料以百分位数法(P_(2.5),P_(97.5))表示,建立正常儿童不同年龄段AMH的医学参考区间。结果男/女新生儿(≤28 d)AMH平均水平分别为83.47、0.14 ng/mL,与出生时间相关性较低;>28 d的男/女童年龄与AMH值明显相关。男童婴儿(>28 d至1岁)、幼儿(>1~3岁)、学龄前(>3~6岁)、学龄期(>6~10岁)及青春期(>10~16岁)的AMH平均水平分别为123.13、145.84、105.88、71.16、26.70 ng/mL(中位数为20.60 ng/mL),女童各年龄段AMH平均水平分别为1.58、1.85、2.53、3.16、2.76 ng/mL。ANOVA检验结果显示,各年龄段AMH结果分布差异有统计学意义(P<0.05)。结论成功建立西南地区不同年龄段儿童的血清AMH医学参考区间,其可为相关疾病诊疗预后提供依据。

Objective To establish the normal reference range of anti-Mullerian hormone(AMH)in healthy children of different ages in Southwest China.Methods Children(0-16 years old)who received healthy physical examination in the hospital from April 2021 to January 2022 were selected as the research objects.The serum AMH level was measured by chemiluminescence immunoassay.After screening,the serum AMH values of 269 qualified children were finally included.The statistical software SPSS 19.0 was used to analyze the data.The reference range of normal distribution measurement data was expressed by 95%confidence interval(95%CI),and the measured data of abnormal distribution were expressed by percentile method(P_(2.5),P_(97.5))so as to establish the medical reference interval of AMH in different age groups of normal children.Results The average AMH levels of male/female newborn were 83.47 and 0.14 ng/mL,respectively,which had a low correlation with the birth time.The age of male/female newborn over 28 days was obviously correlated with AMH value.The mean levels of AMH of boys in infancy(mole than 28 days to one year),toddler(more than one to three years),preschool(more than three to six years),school-age(more than six to ten years)and adolescence(more than 10 to 16 years)were 123.13,145.84,105.88,71.16,26.70 ng/mL(median 20.60 ng/mL).The AMH results of girls in different age groups were 1.58,1.85,2.53,3.16,2.76 ng/mL.The results of analysis of variance(ANOVA)test showed that there were statistically significant differences in the distribution of AMH results among different age groups(P<0.05).Conclusion The medical reference range of serum AMH for children of different ages in Southwest China is snccessfully established,it can provide basis for the diagnosis,treatment and prognosis of related diseases.