美沙拉嗪联合蒲公英多糖治疗溃疡性结肠炎中炎症因子及髓过氧化物酶相关性的研究

Study on the correlation between inflammatory factors and myeloperoxidasein the treatment of ulcerative colitis with mesalasize combined with dandelion polysaccharide

目的:观察蒲公英多糖对溃疡性结肠炎(ulcerative colitis,UC)模型大鼠炎症因子白细胞介素(IL)-6、IL-4、IL-8、IL-10和肿瘤坏死因子-α(TNF-α)水平以及结肠组织中髓过氧化物酶(MPO)水平的影响。方法:采用2,4,6-三硝基苯磺酸复合50%乙醇溶液建立大鼠UC模型,以水提醇沉法提取得到蒲公英精制多糖。50只雄性大鼠随机分为5组:正常对照组、模型对照组、美沙拉嗪组、蒲公英多糖组和美沙拉嗪联合蒲公英多糖治疗组(联合治疗组),每组10只。利用酶联免疫吸附试验检测各组大鼠血清中炎性因子即IL-6、IL-4、IL-8、IL-10、TNF-α的含量,以及结肠组织中MPO表达。结果:与正常对照组比较,模型对照组、蒲公英多糖组血清IL-6、IL-8、TNF-α水平、结肠组织中MPO的含量升高,IL-4和IL-10水平降低(P<0.05),美沙拉嗪组血清IL-6和TNF-α水平升高,IL-4和IL-10水平降低(P<0.05),联合治疗组各因子水平差异无统计学意义(P>0.05);与模型对照组比较,美沙拉嗪组、蒲公英多糖组和联合治疗组血清IL-6、IL-8、TNF-α水平、结肠组织中MPO的含量降低,IL-4和IL-10水平升高(P<0.05);与美沙拉嗪组、蒲公英多糖组比较,联合治疗组血清IL-6、IL-8、TNF-α水平、结肠组织中MPO的含量降低,IL-4和IL-10水平升高(P<0.05)。IL-6、IL-8、TNF-α表达水平和MPO水平呈正相关关系,IL-4和IL-10表达水平和MPO水平呈负相关关系(P<0.05)。结论:美沙拉嗪联合蒲公英多糖治疗UC效果优于单用美沙拉嗪和蒲公英多糖。细胞因子IL-6、IL-8、TNF-α与结肠组织中MPO的水平、炎症的进展呈正相关,而细胞因子IL-4和IL-10与MPO的水平、炎症的进展呈负相关。

Objective:To observe the correlation between the levels of inflammatory factors interleukin(IL)-6,IL-4,IL-8,IL-10,tumor necrosis factor-α(TNF-α)and myeloperoxidase(MPO)in the ulcerative colitis(UC)model rats treated with dandelion polysaccharide.Methods:The UC model of rats was established by 2,4,6-trinitrobenzenesulfonic acid combined with 50%ethanol solution.The refined dandelion polysaccharide was extracted by water extraction and alcohol precipitation method.Fifty male rats were randomly divided into 5 groups:normal control group,model control group,mesalazine group,dandelion polysaccharide group and mesalazine combined with dandelion polysaccharide treatment group(combined treatment group),with 10 rats in each group.The levels of serum inflammatory factors(IL-6,IL-4,IL-8,IL-10,TNF-α)and the expression of MPO in colon tissue were detected by enzyme-linked immunosorbent assay.Results:Compared with the normal control group,the serum levels of IL-6,IL-8 and TNF-αand MPO content in colon tissues were significantly increased,and the levels of IL-4 and IL-10 were decreased in the model control group and dandelion polysaccharide group(P<0.05),meanwhile,the serum levels of IL-6 and TNF-αwere increased and the levels of IL-4 and IL-10 were significantly decreased in the mesalazine group(P<0.05),and there was no significant difference in the levels of each factor in the combined treatment group(P>0.05).Compared with the model control group,the mesalazine group,dandelion polysaccharide group and combination treatment group had significant reductions in the serum levels of IL-6,IL-8,TNF-αand MPO content in colon tissues,and significant increases in the levels of IL-4 and IL-10(P<0.05).Compared with the mesalazine group and the dandelion polysaccharide group,the combined treatment group had significant reductions in the serum levels of IL-6,IL-8,TNF-αand the MPO content in colon tissues,and significant increases in the levels of IL-4 and IL-10(P<0.05).The expression levels of IL-6,IL-8 and TNF-αwere positively correlated with MPO level,and the levels of IL-4 and IL-10 were negatively correlated with the levels of MPO(P<0.05).Conclusions:The effect of mesalazine combined with dandelion polysaccharide in the treatment of UC is better than that of mesalazine and dandelion polysaccharide alone.The levels of IL-6,IL-8 and TNF-αare positively correlated with the levels of MPO and the progression of inflammation,while the levels of IL-4 and IL-10 are negatively correlated with the levels of MPO and the progression of inflammation.