HS-SPME-GC-MS结合化学计量法分析刺五加不同部位的挥发性成分

Analysis of the Volatile Components of Different Parts of Acanthopanax Senticosus by HS-SPME-GC-MS Combined with Chemometric Methods

目的对刺五加不同部位的挥发性成分进行比较分析,为其后续的临床应用及开发提供科学参考。方法采用顶空固相微萃取-气相色谱-质谱联用法(HS-SPME-GC-MS),提取分析刺五加各部位样品的挥发性成分,用峰面积归一化法测定和比对各成分的相对含量,并结合主成分分析(PCA)和聚类分析(HCA)方法分析其成分的差异。结果从刺五加各部位样品中共鉴定出167种挥发性成分,根、皮、茎、叶、果分别鉴定出71、61、59、48、54种挥发性成分,主要以烯类和醇类化合物为主。5个部位有10个共有成分,包括萜品油烯、β-石竹烯、α-律草烯等。HCA分析结果将刺五加根、果聚为一类,皮、叶聚为一类,茎单独一类;PCA综合得分依次为根、茎、果、叶、皮。结论刺五加各部位挥发性成分的种类和含量均有差异,为刺五加非常规药用部位的药食两用深入开发利用提供科学依据。

OBJECTIVE To conduct a comparative analysis of the volatile components of different parts of Acanthopanax senticosus,and provide scientific reference for their subsequent clinical application and development.METHODS Headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry(HS-SPME-GC-MS)was used to extract and analyze the volatile components of the samples of various parts of Acanthopanax senticosus.The relative contents of the components were determined and compared by the peak area normalization method,and the differences of the components were analyzed with principal component analysis(PCA)and hierarchical cluster analysis(HCA).RESULTS A total of 167 volatile components were identified from samples of various parts of Acanthopanax senticosus,with 71,61,59,48 and 54 volatile components identified in the root,velamen,stem,leaf and fruit,respectively,mainly as alkenes and alcohols.10 common components were identified in five parts,including terpinolene,β-staphylene andα-rutinolene,etc.The results of HCA analysis clustered Acanthopanax senticosus root and fruit into one group,velamen and leaf into one group,and stem into a separate group.The PCA scores in the order were:root,stem,fruit,leaf and bark.CONCLUSION The types and contents of volatile components in the various parts of Acanthopanax senticosus are different,providing a scientific basis for the further development and utilization of the unconventional medicinal parts of Acanthopanax senticosus for medicinal and food purposes.