火针疗法对白癜风抗氧化应激能力的作用、皮损面积改善及外周血miR-202-3p、miR-630和nesfatin-1表达的影响

Effect of Fire Needling on Anti-Oxidative Stress,Skin Lesions and Expressions of miR-202-3p,miR-630,nesfatin-1 in Peripheral Blood in Treatment of Vitiligo

目的:探讨火针疗法对白癜风抗氧化应激能力的作用、皮损面积改善及外周血miR-202-3p、miR-630和nesfatin-1表达的影响。方法:选择100例白癜风患者纳入本研究,病例选择样本为2019年10月—2020年10月于本院门诊治疗的患者。将100例白癜风患者分为对照组和研究组,对照组患者行308 nm准分子激光治疗,研究组在激光治疗的基础上行火针治疗。两组患者疗程均为8周,疗程结束后对比两组患者治疗前后皮损面积、靶皮损部位复色率、起效时间、细胞内活性氧(ROS)水平、血清摄食抑制因子(nesfatin-1)以及外周血miRNA-202-3p和miRNA-630表达水平。结果:研究组治疗总有效率96.00%(48/50),明显高于对照组的74.00%(37/50)(P<0.05);疗程结束后两组皮损面积均小于治疗前(P<0.05),研究组皮损面积明显小于对照组(P<0.05),研究组靶皮损部位复色率明显高于对照组(P<0.05),研究组靶皮损部位起效时间明显小于对照组(P<0.05);疗程结束后两组患者miRNA-202-3p和miRNA-630表达水平均高于治疗前(P<0.05),且研究组均明显高于对照组(P<0.05);疗程结束后两组患者血清nesfatin-1水平明显低于治疗前(P<0.05)且研究组明显低于对照组(P<0.05);疗程结束后两组患者细胞内ROS水平明显低于治疗前(P<0.05),且研究组明显低于对照组,差异有统计学意义(P<0.05)。结论:火针疗法对白癜风患者疗效较好,可显著提高患者抗氧化应激能力,减少皮损面积,改善机体免疫功能,降低血清nesfatin-1含量并提高miRNA-202-3p和miRNA-630表达水平。

Objective:To explore the effect of fire needling on anti-oxidative stress,skin lesion area and expressions of miR-202-3p,miR-630 and nesfatin-1 in peripheral blood in treatment of vitiligo.Methods:100 patients with vitiligo were randomly divided into the control group and the study group.The control group received 308 nm excimer laser treatment;on which basis,the study group also received fire needling treatment.The treatment course of the two groups was 8 weeks.Skin lesion area,target skin lesion recolor rate,onset time,level of intracellular reactive oxygen species(ROS),serum content of feeding inhibitor(nesfatin-1),as well as expressions of miRNA-202-3p and miRNA-630 in peripheral blood were compared between before and after the treatment in the two groups.Results:The total effective rate of the study group was 96.00%(48/50),which was significantly higher than 74.00%(37/50)of the control group(P<0.05).The area of skin lesions was smaller after the treatment than that before the treatment in the two groups(P<0.05),which was significantly smaller in the study group than that in the control group(P<0.05).The recolor rate of the target skin lesions in the study group was significantly higher than that of the control group(P<0.05),and the onset time of the target skin lesions in the study group was significantly earlier than that of the control group(P<0.05).The expressions of miRNA-202-3p and miRNA-630 were higher after the treatment than those before the treatment in the two groups(P<0.05),which were significantly higher in the study group than those in the control group after the treatment(P<0.05).The serum level of nesfatin-1 was lower after the treatment than that before the treatment in the two groups(P<0.05),which was lower in the study group than that in the control group(P<0.05).The intracellular ROS level was significantly lower after the treatment than that before the treatment in the two groups(P<0.05),which was significantly lower in the study group than that in the control group after the treatment(P<0.05).Conclusion:Fire needling has a significant effect in treatment of vitiligo.It can significantly improve the patient’s ability to resist oxidative stress,reduce the area of skin lesions,improve the immune function within the body,reduce the serum content of nesfatin-1 and increase the expressions of miRNA-202-3p and miRNA-630.