氮掺杂碳量子点电化学发光检测谷胱甘肽

Determination of glutathione based on electrochemiluminescence of N-CQDs

碳量子点(CQDs)以其优良的光电性能,以及环保、价廉、易得的优点而受到极大关注以柠檬酸(CA)和三聚氰胺(Melamine)为原料,采用一步水热法成功制备了氮掺杂的碳量子点(N-CQDs).对其形貌、组成及光谱性能进行了表征,详细研究了其电化学发光(ECL)性能,结果表明:所合成的N-CQDs在共反应剂过硫酸钾(K2S2O8)存在下能发射强且稳定的ECL信号进一步研究显示:金纳米粒子(Au NPs)的存在会导致N-CQDs的ECL信号猝灭,而谷胱甘肽(GSH)的加入又能使其ECL信号得以恢复.根据这一特点实现了对GSH的灵敏检测,线性响应范围为1.0×10^(-9)~1.0×10^(-4)mol·L^(-1),检出限是8.0×10^(-10)mol·L^(-1).该策略也可用于其他含巯基(-SH)的生物硫醇类物质的检测.

Carbon quantum dots(CQDs) have attracted great attention because of their excellent photoelectric properties,environmental friendliness,low cost,and easy availability.Nitrogen doped carbon quantum dots(N-CQDs) were prepared by one-step hydrothermal method using melamine and citric acid(CA) as raw materials.Their morphology,composition,and spectral properties were characterized with various technologies.The electrochemical luminescence(ECL) property of N-CQDs was investigated in detail.The result showed that the synthesized N-CQDs could emit strong and stable ECL signals in the presence of co-reactive potassium persulfate (K2S2O8).Further studies showed that the presence of gold nanoparticles(Au NPs)could cause the ECL signal of N-CQDs to be quenched,and the addition of glutathione(GSH) could restore the ECL signal.Based on this principle,the sensitive detection of GSH was achieved with a linear response range of 1.0×10^(-9)to 1.0×10^(-4)mol·L^(-1)and a detection limit of 8.0×10^(-10)mol·L^(-1).This strategy can also be used for the detection of other mercapto(-SH) containing biothiols.