沸石咪唑框架纳米复合颗粒对菌膜的清除研究

Study on zeolitic imidazolate framework nanocomposites for biofilm eradication

近年来,新型纳米抗菌材料因其特殊的理化性质和作用机制,在抗菌膜方面展示了不容忽视的潜力.但是,如何提高这些抗菌剂穿透菌膜胞外聚合物基质(EPS)的能力,以达到低剂量、高疗效的目的,仍是一个挑战.为了解决这个难题,通过一锅法成功合成了一种带正电、靶向菌膜中胞外脱氧核糖核酸(eDNA)的沸石咪唑框架纳米复合颗粒(ZIF-8@D).研究发现,ZIF-8@D能够迅速穿透菌膜,并在菌膜内部释放脱氧核糖核酸酶I(DNase I),DNase I能水解菌膜中的eDNA,以达到分散菌膜的目的 .这项研究不仅为设计高穿透性的抗菌膜材料提供了新思路,而且拓展了沸石咪唑框架结构在生物医学领域的应用.

In recent years, novel antibacterial nanomaterials show a non-negligible potential in anti-biofilm due to their specific physiochemical properties and mechanism of action. However,it is still a challenge to improve the ability of these antibacterial agents to penetrate the extracellular polymeric matrix(EPS) of the biofilm to achieve the goal of high efficacy at low dose. To solve this problem,we adopted the one-pot method to successfully synthesize a zeolitic imidazolate framework nanocomposite with positive charge and targeting extracellular deoxyribonucleic acid(eDNA) in the biofilm(ZIF-8@D). The results showed that ZIF-8@D nanoparticles could quickly penetrate the biofilm and release deoxyribonuclease(DNase I) inside the biofilm,which hydrolyzed the eDNA to disperse the biofilm. This work not only provides a new insight into designing anti-biofilm materials with high penetrability,and also expands the application of zeolite imidazole frameworks in biomedicine.