基于HS-SPME-GC-MS分析紫苏挥发油及其包合物挥发性成分

Analysis of The Volatile Components in Volatile Oil of Perilla Folium and Its Inclusion Complex Based on HS-SPME-GC-MS

目的:运用顶空-固相微萃取结合气相色谱-质谱联用技术(HS-SPME-GC-MS)分析紫苏挥发油及其两种环糊精包合物中主要挥发性成分,并比较其成分与相对含量差异。方法:水蒸气蒸馏法提取紫苏挥发油,采用饱和水溶液法制备其β-环糊精(β-CD)及羟丙基-β-环糊精(HP-β-CD)包合物,通过HS-SPME提取挥发性成分后利用GC-MS对其挥发性成分进行分析。结果:紫苏挥发油中共鉴定出11个主要成分,占挥发油总量的89.15%,采用β-CD与HP-β-CD制备的紫苏挥发油包合物中分别鉴定出7个和10个主要挥发性成分,分别占总峰面积的74.20%和94.94%,主要成分紫苏醛的相对含量在β-CD与HP-β-CD包合物中分别增加至52.13%和78.78%。结论:运用HS-SPME-GC-MS揭示了β-CD与HP-β-CD包合的紫苏挥发油成分及相对含量具有一定差异,挥发油中的成分被选择性的包合,其中HP-β-CD对紫苏挥发油成分的综合包合效果更好。研究结果为紫苏挥发油在环糊精类包材的选择及包合效果评价提供有益参考。

Objective:To analyze the main volatile components of volatile oil from Perilla Folium and its two cyclodextrin inclusions by HS-SPME-GC-MS,and to compare the composition and relative content differences.Methods:The volatile oil of Perilla Folium was extracted by steam distillation, and the β-cyclodextrin(β-CD) and hydroxypropyl-β-cyclodextrin(HP-β-CD) inclusion complexes of volatile oil from Perilla Folium were prepared by saturated aqueous solution method.The volatile components of volatile oil from Perilla Folium and its two cyclodextrin inclusions were analyzed by GC-MS after concentrated by HS-SPME.Results:A total of 11 major components were identified in the volatile oil, accounting for 89.15% of the total volatile oil.7 and 10 main volatile components were identified in the inclusions of the volatile oil by using β-CD and HP-β-CD,accounting for 74.20% and 94.94% of the total peak area, respectively.The relative content of perilla aldehyde increased to 52.13% and 78.78% in the β-CD and HP-β-CD inclusion complexes, respectively.Conclusion:The components and relative contents of volatile oils from Perilla Folium encapsulated by β-CD and HP-β-CD were different.The components in volatile oils were selectively encapsulated, and HP-β-CD had better comprehensive inclusion effect on volatile oil.This study provided useful references for the selection of the volatile oil from Perilla Folium in cyclodextrin-based package materials and the evaluation of the encapsulation effect.