基于方波伏安法的聚多巴胺@纳米银修饰玻碳电极测定畜禽肉中氯丙嗪

Determination of Chlorpromazine in Livestock and Poultry Meat by Polydopamine@Nanosilver Modified Glassy Carbon Electrode Based on Square Wave Voltammetry

基于多巴胺(dopamine,DA)的自聚反应,在纳米银(sliver nanoparticles,AgNPs)和玻碳电极(glassy carbon electrode,GCE)表面形成聚多巴胺(polydopamine,PDA)膜,使AgNPs均匀分散在GCE表面,得到PDA@AgNPs/GCE修饰电极;对DA的自聚时间、氯丙嗪的电化学测试方法及条件等进行考察和优化,构建基于方波伏安法的氯丙嗪快速检测方法;利用循环伏安法、交流阻抗法和扫描电子显微镜对修饰电极进行表征。最佳条件下,氯丙嗪在PDA@AgNPs/GCE上的氧化峰电流与其浓度在5.0×10^(-8)~1.0×10^(-5)mol/L范围内呈良好的线性关系(R^(2)=0.991),检出限(R_(SN)=3)为1.7×10^(-8)mol/L。用该法测定猪肉、鸡肉和牛肉中氯丙嗪的加标回收率分别为84.9%~88.8%、79.8%~93.8%、80.6%~93.9%。该方法所用测试仪器轻巧便携,修饰电极的制备方法简便易行、成本低、便于批量制备,可用于畜禽肉中氯丙嗪的快速检测。

Based on the self-polymerization reaction of dopamine(DA),a polydopamine(PDA)film was formed on the surface of silver nanoparticles(AgNPs)and glassy carbon electrode(GCE),which could result in uniform dispersion of AgNPs on the surface of GCE.As a result,PDA@AgNPs/GCE modified electrode was obtained.The self-polymerization time of DA and the electrochemical test method and conditions for chlorpromazine were investigated and optimized to establish a rapid method for the detection of chlorpromazine based on square wave voltammetry.The modified electrode was characterized by cyclic voltammetry(CV),electrochemical impedance spectroscopy(EIS)and scanning electron microscopy(SEM).Under the optimal conditions,the oxidation peak current of chlorpromazine on PDA@AgNPs/GCE had a good linear relationship with its concentration in the range of 5.0×10^(-8)–1.0×10^(-5)mol/L(R^(2)=0.991).The detection limit(R_(SN)=3)was1.7×10^(-8)mol/L.The recoveries of chlorpromazine in spiked pork,chicken and beef samples were 84.9%–88.8%,79.8%–93.8%and 80.6%–93.9%,respectively.The instrument used in the method was light and portable,and the preparation method of the modified electrode was simple,easy,cheap,and convenient for batch preparation of modified electrodes,and could be used for the rapid detection of chlorpromazine in livestock and poultry meat.