TNNI1、TNNI3在麦洼牦牛横纹肌中的表达分析

Expression Analysis of TNNI1 and TNNI3 in Striated Muscle of Maiwa Yak

为探索TNNI1、TNNI3在麦洼牦牛横纹肌中的表达模式,选取不同年龄麦洼牦牛臀肌、心肌组织,观察其形态学变化,开展了不同年龄麦洼牦牛TNNI1基因在臀肌组织中的表达趋势及与肌纤维性状的关联分析,同时对TNNI1、TNNI3在心肌中的mRNA和蛋白差异表达及蛋白定位进行了相关性分析。结果表明,随着牦牛年龄增长,肌纤维直径及密度存在一定特征性变化,其中肌纤维直径随年龄的增长显著增加,肌纤维密度则发生相反的变化。成年牦牛TNNI1基因在臀肌组织中的mRNA表达水平显著高于犊牛及断奶犊牛,变化趋势具体为成年牛>犊牛>断奶犊牛;且臀肌中TNNI1 mRNA表达水平与肌纤维直径极显著正相关,与肌纤维密度显著负相关。TNNI1在心肌中的mRNA表达水平随牦牛年龄的增大而降低,而TNNI3则表现为先升高后降低的趋势,且TNNI1在犊牛期mRNA表达水平最高,TNNI3则随年龄增长呈上调趋势。TNNI1、TNNI3蛋白在心肌组织中的表达量随牦牛年龄的增长逐渐减小,且TNNI3 mRNA表达量随牦牛年龄的增长逐渐降低,与蛋白表达趋势一致。TNNI1、TNNI3对不同年龄麦洼牦牛肌肉组织的生长发育具有显著调控作用,且在不同年龄牦牛肌肉组织中的表达水平存在差异。

In order to explore the expression patterns of TNNI1 and TNNI3 in the striated muscle of Maiwa yak,this study selectd the gluteal muscle and myocardial tissue of Maiwa yak of different ages,morphological changes observed.The expression trend of TNNI1 gene in gluteal muscle tissue of Maiwa yak at different ages and its association with muscle fiber traits were carried out,the mRNA,protein differential expression and protein localization of TNNI1 and TNNI3 in myocardium were analyzed.The results showed that the muscle fiber diameter and density had certain characteristic changes with the increase of yak age.The muscle fiber diameter increased significantly with the increase of age,while the muscle fiber density had the opposite change.The mRNA expression of TNNI1 gene in gluteal muscle tissue of adult yaks was significantly higher than calves and weaned calves.TNNI1 mRNA expression in gluteus muscle was significantly positively correlated with muscle fiber diameter,and significantly negatively correlated with muscle fiber density.The mRNA expression level of TNNI1 in myocardium decreased with the increase of yak age,while the expression level of TNNI3 showed a trend of first increased and then decreased,and the mRNA expression level of TNNI1 was the highest in calf stage,while TNNI3 showed an upward trend with the increase of age.The expression levels of TNNI1 and TNNI3 proteins in myocardium decreased with the increase of yak age.The expression levels of TNNI1 and TNNI3 proteins in myocardium decreased with the increase of yak age.The TNNI3 mRNA expression decreased with the increase of yak age,which was consistent with the trend of protein expression.It provided basic data for exploring the regulation mechanism of TNNI1 and TNNI3 on yak muscle growth and development.TNNI1 and TNNI3 have significant regulatory effects on the growth and development of the muscle tissue of Maiwa yak at different ages,and the expression levels in the muscle tissue of yak at different ages are different.