基于3种色谱质谱联用技术的南葶苈子和北葶苈子脂质成分分析

Analysis of the lipids of Descurainiae Semen and Lepidii Semen based on three chromatography tandem mass spectrometry techniques

目的阐明南葶苈子Descurainiae Semen和北葶苈子Lepidii Semen中脂质成分的物质基础,并比较基于反相色谱串联四极杆飞行时间质谱(reverse phase liquid chromatography tandem quadrupole time of flight mass spectrometry,RPLC-Q-TOFMS)、超高效合相色谱串联四极杆飞行时间质谱(ultra performance convergence chromatography-Q-TOF-MS,UPCC-Q-TOFMS)与亲水相互作用色谱串联四极杆飞行时间质谱(hydrophilic interaction liquid chromatography-Q-TOF-MS,HILIC-Q-TOFMS)3种技术对脂质成分的鉴定效果。方法采用RPLC、UPCC与HILIC 3种分离模式分别串联Q-TOF-MS深入解析种子类中药南葶苈子和北葶苈子中的脂质成分。以Waters Acquity HSS T3(100 mm×2.1 mm,1.8μm)为反相色谱柱,Waters ACQUITY UPCC®TORUS 2-PIC(100 mm×3.0 mm,1.7μm)为合相色谱柱,Waters BEH HILIC(100 mm×2.1 mm,1.7μm)为亲水相互作用色谱柱,在正离子模式下采集南葶苈子和北葶苈子中脂质成分的质谱数据。通过MS Dial软件Lipidomics数据库自动匹配并进行人工确认,实现对南葶苈子和北葶苈子中脂质成分的分析和鉴定。结果共鉴定出191个脂质成分,包括三酰甘油、甘油二酯、磷脂酰乙醇胺、溶血磷脂酰乙醇胺、磷脂酰胆碱、溶血磷脂酰胆碱和磷脂酰肌醇。结论阐明了南葶苈子和北葶苈子中脂质成分的物质基础,为南葶苈子和北葶苈子的临床应用、质量控制及制剂开发提供实验依据,也为种子类中药中脂质成分的分析提供参考。

Objective To elucidate the material basis of lipids in Nantinglizi(Descurainiae Semen)and Beitinglizi(Lepidii Semen),and compare the identification effects of lipids based on reverse phase liquid chromatography tandem quadrupole time of flight mass spectrometry(RPLC-Q-TOF-MS),ultra performance convergence chromatography-Q-TOF-MS(UPCC-Q-TOF-MS)and hydrophilic interaction liquid chromatography-Q-TOF-MS(HILIC-Q-TOF-MS)techniques.Methods The lipids of Descurainiae Semen and Lepidii Semen were analyzed by RPLC,UPCC and HILIC coupled with Q-TOF-MS.RPLC separations of Descurainiae Semen and Lepidii Semen were performed on Waters Acquity HSS T3(100 mm×2.1 mm,1.8μm).UPCC separations were on Waters ACQUITY UPCC®TORUS 2-PIC(100 mm×3.0 mm,1.7μm).HILIC separations were on Waters BEH HILIC(100 mm×2.1 mm,1.7μm)and the data was acquired in positive ESI mode.The lipids were analyzed and identified by automatically matching the Lipidomics database of MS Dial software and manual confirmation.Results A total of 191 lipids were characterized,including triacylglycerols,diacylglycerols,phosphatidylethanolamines,lysophosphatidylethanolamines,phosphatidylcholines,lysophosphatidylcholines and phosphatidylinositols.Conclusions The results provided experimental evidences for the clinical application,quality control and preparation development of Descurainiae Semen and Lepidii Semen,along with a reference for the analysis of lipids in the Chinese medicines of seeds.