特染硼酸亚甲蓝法与13C-尿素呼气试验法对幽门螺杆菌检测效果比较

Comparative study of specific staining and 13C-urea breath test in the detection of Helicobacter pylori

目的 探讨特染硼酸亚甲蓝法(特染法)与13C-尿素呼气试验(UBT)法在幽门螺杆菌(HP)检测中的应用效果。方法 选取2020年5—12月芜湖市第一人民医院收治的149例患者为研究对象。所有患者行胃镜检查前均进行了13C-UBT法检测HP;胃镜活检后标本均进行了特殊染色以识别活检标本中的HP。比较两种方法的HP阳性检出率。结果 149例患者中,13C-UBT法检测HP阳性且特染法检测HP阴性7例,13C-UBT法检测HP阴性且特染法检测HP阳性68例。149例患者中,13C-UBT检测HP阳性率为26.85%(40/149),低于特染法的67.79%(101/149),差异有统计学意义(P<0.05)。在诊断伴有肠化生的64例患者中,13C-UBT检测HP阳性率为31.25%(20/64),低于特染法的65.63%(42/64),差异有统计学意义(P<0.05)。结论 特染法检测HP的效果更优于13C-UBT法。两种方法同时应用能更加准确地进行HP的检测,更有效地指导临床用药治疗。

Objective To investigate the application effect of methylene borate blue method and13C-urea breath test(UBT)in the detection of helicobacter pylori(HP).Methods A total of 149 patients admitted to The First People′s Hospital of Wuhu from May to December 2020 were selected as the research objects.HP was detected by13C-UBT before gastroscopy in all patients.Special staining was performed after gastroscopic biopsy to identify HP in biopsy specimens.The positive detection rate of HP was compared between the two methods.Results Among the 149 patients, 7 were HP positive and negative by13C-UBT method, and 68 were HP negative and positive by13C-UBT method.Among 149 patients, the positive rate of HP detected by13C-UBT was 26.85%(40/149),lower than 67.79%(101/149)by special dyeing method, and the difference was statistically significant(P<0.05).Among 64 patients diagnosed with intestinal metaplasia, the positive rate of HP detected by13C-UBT was 31.25%(20/64),lower than 65.63%(42/64)by special dye method, and the difference was statistically significant(P<0.05).Conclusion The effect of special dye method was better than that of13C-UBT method.The simultaneous application of the two methods can more accurately detect HP and more effectively guide clinical drug treatment.