壳寡糖复合固体饮料对高尿酸血症小鼠的降尿酸作用研究

Effect of chitooligosaccharide compound solid beverage on reducing uric acid in hyperuricemia mice

为探究壳寡糖复合固体饮料“壳酸平”(ke suan ping,KSP)在高尿酸血症小鼠中的降尿酸及肝肾保护作用,该研究将60只昆明小鼠分为6组:阴性对照组(negative group,NC)、高尿酸血症模型组(model group,M)、低剂量KSP组(low-dose KSP group,LKSP)、中剂量KSP组(middle-dose KSP group,MKSP)、高剂量KSP组(high-dose KSP group,HKSP)、别嘌呤醇组(allopurinol group,AP)。适应性喂养1周后,通过腹腔注射氧嗪酸钾辅以灌胃酵母膏建立了高尿酸血症模型小鼠,同时更换KSP组小鼠饲料为含不同剂量KSP的饲料,AP组在造模1 h以后给药。收集小鼠血清用于尿酸、肌酐、尿素氮、黄嘌呤氧化酶的检测;收集小鼠肝、肾、肠用于苏木精-伊红染色法(hematoxylin-eosin staining,HE染色)和q-PCR。结果显示,与M组相比,KSP可以降低高尿酸血症小鼠血清尿酸、肌酐、尿素氮、黄嘌呤氧化酶活性,缓解高尿酸血症小鼠肝肾损伤,调节GLUT9、URAT1、ABCG2蛋白转录水平的表达。研究证明KSP可以通过降低黄嘌呤氧化酶的活性促进尿酸排泄等发挥降尿酸作用。

To explore the uric acid-lowering effect of chitooligosaccharide solid beverage(ke suan ping,KSP)in hyperuricemia mice,a total of 60 Kunming mice were divided into 6 groups,including a negative control group(NC),hyperuricemia model group(M),and low-dose KSP group(LKSP),middle-dose KSP group(MKSP),high-dose KSP group(HKSP),and allopurinol group(AP).After one week of adaptive feeding,hyperuricemia mice were injected with potassium oxonate and supplemented with yeast extracts.At the same time,the fodders of mice in KSP groups were changed into the forages containing KSP in different doses.The mice in the AP group were administered with PBS buffer after injection.The test lasted 21 days.Serum of mice was collected for the detection of uric acid(UA),creatinine(CR),urine urea nitrogen(BUN),and xanthine oxidase(XOD).And the mouse liver,kidney,and small intestine were collected for HE staining and q-PCR.Results showed that KSP could reduce serum UA,CR,and BUN and the activities of XOD in hyperuricemia mice,alleviate the damage of the liver and kidney in hyperuricemia mice,and regulate the mRNA expression of GLUT9,URAT1,and ABCG2.In short,KSP could reduce UA by inhibiting the activity of XOD and promoting the excretion of UA in hyperuricemia.