摘要
该文探讨二十碳五烯酸、二十二碳六烯酸联合麦芽糊精(maltodextrin,MED)膳食补充对Ⅱ型糖尿病大鼠肠道菌群、血液有机酸及脏器脂分布的影响。构建大鼠Ⅱ型糖尿病模型,以不同剂量MED灌胃患病大鼠,观察其对患病大鼠血糖水平、脏器指数及脏器脂类蓄积的影响,收集实验大鼠血液和粪便,利用高效液相色谱法检测大鼠血液的有机酸含量,通过16S rDNA扩增子测序获取各实验组肠道菌群的信息数据。MED灌胃尤其中剂量对糖尿病引起的多饮症状有一定的缓解作用,对于糖尿病导致的大鼠各器官脏器系数的增大有较好的改善趋势,但会引起脂类在心肝脾肺肾中的蓄积。对于糖尿病引起的肠道菌群的改变,中剂量MED有恢复某些优势有益菌群的相对丰度的趋势。MED可在一定趋势上缓解糖尿病引起的血液有机酸(如丙酸、丁酸、异戊酸和正戊酸)的变化。MED对Ⅱ型糖尿病引起的多饮、高血糖、脏器系数以及肠道菌群的改变有一定缓解作用,但会引起脂类在各脏器的蓄积,应谨慎使用。
The effects of eicosapentaenoic acid(EPA)and docosahexaenoic acid(DHA)combined with maltodextrin(MED)dietary on the gut flora,blood organic acids and visceral distribution of lipids in typeⅡdiabetic rats were investigated.SD rat diabetes model was established by intraperitoneal administration of streptozotocin and oral administration of different doses of MED.The fasting blood glucose,visceral coefficient and lipid visceral distribution were detected.The blood and feces of the subject rats were collected for the blood organic acid detection(by HPLC),and for 16S rDNA amplicon sequencing analysis to obtain the information of intestinal flora in diabetic rat.The results showed that MED gavage,especially in middle dosage,had a certain alleviation effect on polydipsia and the augment of organ coefficient induced by diabetes mellitus but would cause lipids accumulation in solid organs.For the changes of intestinal flora induced by diabetes,middle-dose MED tended to restore the relative abundance of some dominant beneficial flora.MED could in some extent alleviate the diabetes-triggered alteration of blood organic acids as propionic acid,butanoic acid,isopentanoic acid and n-pentanoic acid.MED could alleviate the alteration of polydipsia,hyperglycemia,organ coefficient and intestinal flora caused by typeⅡdiabetes(no statistical significance),but would induce lipids accumulation in visceral organs,Hence,it should be applied with caution.
作者
蒋变玲
王维维
赵程洋
张东新
赵旭东
陈军
彭磊
陈琼
冯凡
JIANG Bianling;WANG Weiwei;ZHAO Chengyang;ZHANG Dongxin;ZHAO Xudong;CHEN Jun;PENG Lei;CHEN Qiong;FENG Fan(School of Biological and Food Engineering,Suzhou University,Suzhou 234000,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2023年第4期202-208,共7页
Food and Fermentation Industries
基金
宿州学院校级科研平台开放课题(2022ykf02)
宿州学院博士科研启动资金(2019jb22,2021BSK021)
宿州市科技公关计划项目(SZSKJJZC001)
宿州学院2021校级科研平台——分子营养与生命健康研究中心(2021XJPT34)
国家级和省级大学生创新创业训练项目(202210379026,S202110379116)
宿州学院科研发展基金项目(2021fzjj03)
宿州市科技计划项目(2019087)
安徽省高等学校科学研究项目(2022AH051362)。
关键词
二十碳五烯酸
二十二碳六烯酸
麦芽糊精
糖尿病
肠道菌群
有机酸
脂类分布
eicosapentaenoic acid
docosahexaenoic acid
maltodextrin
diabetes
intestinal flora
organic acids
lipids distribution