二十碳五烯酸、二十二碳六烯酸联合麦芽糊精对Ⅱ型糖尿病大鼠肠道菌群、有机酸及脂类分布的影响

Effect of eicosapentaenoic acid and docosahexaenoic acid combined with maltodextrin dietary on gut microflora,blood organic acids,and lipids distribution in diabetic rats

该文探讨二十碳五烯酸、二十二碳六烯酸联合麦芽糊精(maltodextrin,MED)膳食补充对Ⅱ型糖尿病大鼠肠道菌群、血液有机酸及脏器脂分布的影响。构建大鼠Ⅱ型糖尿病模型,以不同剂量MED灌胃患病大鼠,观察其对患病大鼠血糖水平、脏器指数及脏器脂类蓄积的影响,收集实验大鼠血液和粪便,利用高效液相色谱法检测大鼠血液的有机酸含量,通过16S rDNA扩增子测序获取各实验组肠道菌群的信息数据。MED灌胃尤其中剂量对糖尿病引起的多饮症状有一定的缓解作用,对于糖尿病导致的大鼠各器官脏器系数的增大有较好的改善趋势,但会引起脂类在心肝脾肺肾中的蓄积。对于糖尿病引起的肠道菌群的改变,中剂量MED有恢复某些优势有益菌群的相对丰度的趋势。MED可在一定趋势上缓解糖尿病引起的血液有机酸(如丙酸、丁酸、异戊酸和正戊酸)的变化。MED对Ⅱ型糖尿病引起的多饮、高血糖、脏器系数以及肠道菌群的改变有一定缓解作用,但会引起脂类在各脏器的蓄积,应谨慎使用。

The effects of eicosapentaenoic acid(EPA)and docosahexaenoic acid(DHA)combined with maltodextrin(MED)dietary on the gut flora,blood organic acids and visceral distribution of lipids in typeⅡdiabetic rats were investigated.SD rat diabetes model was established by intraperitoneal administration of streptozotocin and oral administration of different doses of MED.The fasting blood glucose,visceral coefficient and lipid visceral distribution were detected.The blood and feces of the subject rats were collected for the blood organic acid detection(by HPLC),and for 16S rDNA amplicon sequencing analysis to obtain the information of intestinal flora in diabetic rat.The results showed that MED gavage,especially in middle dosage,had a certain alleviation effect on polydipsia and the augment of organ coefficient induced by diabetes mellitus but would cause lipids accumulation in solid organs.For the changes of intestinal flora induced by diabetes,middle-dose MED tended to restore the relative abundance of some dominant beneficial flora.MED could in some extent alleviate the diabetes-triggered alteration of blood organic acids as propionic acid,butanoic acid,isopentanoic acid and n-pentanoic acid.MED could alleviate the alteration of polydipsia,hyperglycemia,organ coefficient and intestinal flora caused by typeⅡdiabetes(no statistical significance),but would induce lipids accumulation in visceral organs,Hence,it should be applied with caution.