PD-L1和PD-1在合并血吸虫肠病结直肠癌组织中的表达及意义

Expression and significance of programmed death ligand 1 and programmed death 1 in colorectal cancer complicated by schistosomiasis

目的:探讨程序性死亡配体1(PD-L1)和程序性死亡分子1(PD-1)在合并血吸虫肠病结直肠癌组织中的表达及临床意义。方法:收集宣城市人民医院2014—2021年结直肠癌患者手术切除标本134例,其中合并血吸虫肠病74例为病例组,非合并血吸虫肠病60例为对照组。采用免疫组化法检测PD-L1和PD-1在结直肠癌组织中的表达情况,分析组间表达差异及其与各临床病理特征的关系。结果:病例组癌细胞PD-L1、癌间质淋巴细胞PD-1阳性表达率分别为55.4%、60.8%,对照组癌细胞PD-L1、癌间质淋巴细胞PD-1阳性表达率分别为35.0%、40.0%,病例组PD-L1、PD-1阳性表达率均高于对照组,差异均有统计学意义(χ^(2)=5.55、5.74,均 P < 0.05);病例组PD-L1、PD-1表达与癌淋巴结转移及高TNM分期相关( P < 0.05)。 结论:PD-L1和PD-1在合并血吸虫肠病结直肠癌组织中高表达,并与其侵袭性行为有相关性,PD-1/PD-L1信号途径可能参与合并血吸虫肠病结直肠癌的发生、发展分子学机制,阻断PD-1/PD-L1信号通路有望成为合并血吸虫肠病结直肠癌免疫治疗的新策略。

Objective To investigate the expression and significance of programmed death ligand 1(PD-L1)and programmed death 1(PD-1)in colorectal cancer complicated by schistosomiasis.Methods A total of 134 patients with colorectal cancer who received treatment in Xuancheng People's Hospital during 2014-2021 were included in this study.These patients consisted of 74 patients with colorectal cancer combined with schistosomiasis(patient group)and 60 patients with only colorectal cancer(control group).The expression of PD-L1 and PD-1 in colorectal cancer tissue was detected by an immunohistochemical method.The differences in PD-L1 and PD-1 expression were compared between the two groups.The relationships between PD-L1 and PD-1 expression and clinical pathological characteristics were determined.Results The positive expression rates of PD-L1 and PD-1 in cancer cells and interstitial lymphocytes were 55.4%and 60.8%respectively in the patient group and they were 35.0%and 40.0%respectively in the control group.The positive expression rates of PD-L1 and PD-1 were significantly higher in the patient group than the control group(χ^(2)=5.55,5.74,both P<0.05).The expressions of PD-L1 and PD-1 in the patient group were correlated with lymph node metastasis and high tumor-node-metastasis stage(P<0.05).Conclusion PD-L1 and PD-1 are highly expressed in colorectal cancer complicated by schistosomiasis and are related to their invasive behavior.PD-1/PD-L1 singaling pathway may be involved in the molecular mechanism underlying the occurrence and development of colorectal cancer complicated by schistosomiasis.Blocking PD-1/PD-L1 signaling pathway may be a new strategy for immunotherapy of colorectal cancer complicated by schistosomiasis.