敲低PRPF19对胰腺癌细胞增殖、迁移和侵袭的影响

Effects of PRPF19 Knockdown on Proliferation,Migration and Invasion of Pancreatic Cancer Cells

目的探讨敲低PRPF19之后对胰腺癌细胞增殖、迁移和侵袭能力的影响。方法利用GEPIA等数据库分析PRPF19在胰腺癌及其正常组织中的表达差异;通过Western blot和qRT-PCR检测胰腺癌细胞中PRPF19蛋白和mRNA的表达水平;小干扰RNA(siRNA)技术沉默胰腺癌细胞中PRPF19的表达,并通过Western blot和qRT-PCR验证其敲低效率;CCK-8、克隆形成以及Transwell实验检测敲低PRPF19对胰腺癌细胞增殖、克隆形成以及迁移和侵袭能力的影响。结果GEPIA分析显示,与正常胰腺组织相比,PRPF19在胰腺癌组织中高表达;与正常胰腺细胞相比,PRPF19在MIA PaCa-2、PANC-1等多种胰腺癌细胞系中高表达(P<0.05);与对照组相比,敲低PRPF19后,胰腺癌细胞的增殖速率明显下降,克隆形成数量、细胞迁移和侵袭数量均减少(P<0.05)。结论敲低PRPF19可抑制胰腺癌细胞的增殖、迁移和侵袭能力,PRPF19可能作为胰腺癌的一个癌基因发挥重要作用。

Objective To investigate the effects of PRPF19 knockdown on the proliferation,migration,and invasion of pancreatic cancer cells.Methods The expression of PRPF19 in pancreatic cancer and normal tissues was analyzed using the GEPIA database.The protein and mRNA expression levels of PRPF19 in pancreatic cancer cells were detected by Western blot and qRT-PCR.Small interfering RNA(siRNA)was used to silence the expression of PRPF19 in pancreatic cancer cells,and the knockdown efficiency was verified by Western blot and qRT-PCR.CCK-8,colony forming,and Transwell assay were used to detect the effects of knockdown of PRPF19 on the proliferation,colony forming,migration,and invasion of pancreatic cancer cells.Results GEPIA analysis showed that PRPF19 was highly expressed in pancreatic cancer tissues compared with normal pancreatic tissues.In comparison with normal pancreatic cells,PRPF19 was highly expressed in various pancreatic cancer cell lines such as MIA PaCa-2 and PANC-1(P<0.05).In comparison with the control group,PRPF19 knockdown significantly reduced the proliferation rate,colony forming,cell migration,and invasion of pancreatic cancer cells(P<0.05).Conclusion PRPF19 knockdown inhibits the proliferation,migration,and invasion of pancreatic cancer cells.PRPF19 may play an important role as an oncogene of pancreatic cancer.