牛奶子枝叶的化学成分

Chemical Constituents of the Twigs and Leaves of Elaeagnus Umbellata Thunb

为研究牛奶子(Elaeagnus umbellata Thunb)的药效物质基础,利用硅胶柱色谱、Sephadex LH-20凝胶柱色谱、MCI柱色谱及半制备高效液相色谱对其枝叶化学成分进行分离纯化,结合理化性质和波谱数据对所得化合物进行结构鉴定.从牛奶子枝叶中分离得到16个化合物,分别鉴定为:4-羟基-4-(3-氧代-1-丁烯基)-3,5,5-三甲基环己-2-烯-1-酮(1),(3S,5R,6S,7E)-5,6-环氧-3-羟基-7-大柱香波龙烯-9-酮(2),(6R,7E,9R)-9-羟基-4,7-大柱香波龙二烯-3-酮(3),黑麦草内酯(4),2α,3α,23-三羟基-12-烯-28-熊果酸(5),2α,3α,23-三羟基-3,23-异亚丙基二氧基乌苏-12-烯-28-酸(6),2α-羟基熊果酸(7),熊果酸(8),白桦脂酸(9),3-羟基-1-(3-羟基-4-甲氧基苯)-1-丙酮(10),pinoresinol(11),丁香脂素(12),lariciresinol(13),紫云英苷(14),β-谷甾醇(15),胡萝卜苷(16).除化合物14和15外,其余化合物皆为首次从该植物中分离得到.

To study the chemical constituents of the twigs and leaves of Elaeagnus umbellata Thunb,the ethyl acetate extraction was isolated and purified by column chromatography over silica gel,Sephadex LH-20,MCI gel,as well as on semi-preparative HPLC.The structures of the obtained compounds were identified through NMR spectral data.Sixteen compounds were isolated and identified to be:4-hydroxy-4-(3-oxo-1-butenyl)-3,5,5-tri methylcyclohex-2-en-1-one(1),(3S,5R,6S,7E)-5,6-epoxy-3-hydroxy-7-megastigmen-9-one(2),(6R,7E,9R)-9-hydroxyl-4,7-megastigmadien-3-one(3),loliolide(4),2α,3α,23-trihydroxyurs-12-en-28-oic acid(5),2α,3α,23-trihydroxy-3α,23-isopropylidenedioxyurs-12-en-28-oic acid(6),2α-hydroxyursolic acid(7),ursolic acid(8),betulinic acid(9),3-hydroxy-1-(3-hydroxy-4-methoxyphenyl)propan-1-one(10),pinoresinol(11),syringaresinol(12),lariciresinol(13),astragalin(14),β-sitosterol(15)and daucosterol(16).Except for compounds 14 and 15,all the other compounds were isolated from this plant for the first time.