摘要
目的 筛选具有保护人皮肤角质形成细胞(HACAT)抗过氧化氢(H_(2)O_(2))氧化损伤的活性样品,并探索其抗氧化的机制。方法 用CCK-8法测定不同植物样品对H_(2)O_(2)损伤HACAT细胞的保护作用,获得活性样品远志寡精F。检测远志寡精F对损伤细胞的增殖活性、超氧化物歧化酶(SOD)及谷胱甘肽过氧化物酶(GSH-Px)的活性、丙二醛(MDA)、活性氧(ROS)、白细胞介素(IL)-6、IL-8的含量以及细胞凋亡和坏死的影响。结果 经筛选得到的活性物质远志寡精F能提高损伤细胞的增殖活力、SOD及GSH-Px活性,减少MDA、ROS的产生和IL-6、IL-8的表达,降低细胞的凋亡率和坏死率。结论 远志寡精F可能是通过增加抗氧化活性物质的产生、减轻炎症反应、减少细胞凋亡和坏死来发挥抗氧化损伤的作用。
OBJECTIVE To screen the active samples which can protect HACAT cell against H_(2)O_(2)oxidative damage, and to explore its mechanism.METHODS The protective effects of different plant samples on H_(2)O_(2)damaged HACAT cells were determined by CCK-8 to obtain the active samples.The effect of active samples on proliferation, activities of SOD and GSH-Px, contents of MDA,ROS,IL-6 and IL-8,apoptosis and necrosis of injured cells were detected.RESULTS Tenuifolioses F was obtained by screening.It can increase the proliferation, activities of SOD and GSH-Px, decrease the levels of MDA,ROS,IL-6 and IL-8,reduce the apoptosis and necrosis of injured HACAT cells.CONClUSION Tenuifolioses F may play an anti-oxidative injury role by increasing antioxidant active substances, reducing inflammatory response, and reducing cell apoptosis and necrosis.
作者
李昕洁
杨彬彬
汪婕
潘源峙
喻凯
王明奎
LI Xinjie;YANG Binbin;WANG Jie;PAN Yuanzhi;YU Kai;WANG Mingkui(School of Life Science and Engineering,Southwest Jiaotong University,Chengdu,Sichuan,610031 P.R.China;Chengdu Institute of Biology,Chinese Academy of Sciences,Chengdu,Sichuan,610041 P.R.China)
出处
《华西药学杂志》
CAS
CSCD
2023年第1期43-46,共4页
West China Journal of Pharmaceutical Sciences
基金
四川省科技计划项目(2021ZHFP0031)。