摘要
Retinal pigment epithelium(RPE)has essential functions,such as nourishing and supporting the neural retina,and is of vital importance in the pathogenesis of age-related retinal degeneration.However,the exact molecular changes of RPE during aging remain poorly understood.Here,we isolated human primary RPE(h RPE)cells from 18 eye donors distributed over a wide age range(10–67 years old).A quantitative proteomic analysis was performed to analyze changes in their intracellular and secreted proteins.Age-group related subtypes and age-associated proteins were revealed and potential age-associated mechanisms were validated in ARPE-19 and h RPE cells.The results of proteomic data analysis and verifications suggest that RNF123-and RNF149-related protein ubiquitination plays an important role in protecting h RPE cells from oxidative damage during aging.In older h RPE cells,apoptotic signaling-related pathways were up-regulated,and endoplasmic reticulum organization was down-regulated both in the intracellular and secreted proteomes.Our work paints a detailed molecular picture of h RPE cells during the aging process and provides new insights into the molecular characteristics of RPE during aging and under other related clinical retinal conditions.
基金
supported by grants from the National Natural Science Foundation of China(Grant Nos.82004001 and 82071008)
the Science and Technology Department of Henan Province,China(Grant Nos.212102310307 and 192102310076)。
