铜绿丽金龟线粒体全基因组及其系统发育分析

Whole Mitochondrial Genome and Phylogeny Analysis of Anomala corpulenta

铜绿丽金龟Anomala corpulenta是一种重要的农林害虫。本文采用Illumina HiSeq X平台对其进行线粒体基因组测序,对基因组序列进行拼装、注释,并对线粒体基因组结构特征和碱基组成进行分析。基于13个蛋白质编码基因的核苷酸序列,采用最大似然法和贝叶斯法构建金龟科系统发育树。结果表明,铜绿丽金龟线粒体基因组全长为16 673 bp,包括13个蛋白质编码基因、22个tRNA基因、2个rRNA基因和一段长约1 074 bp的A+T富集区。基因排布与已知的其他丽金龟科昆虫完全相同,且遵循祖先模式,未发生基因重排。系统发育分析显示,丽金龟亚科的所有物种聚在同一分支,支持该亚科的单系性;异丽金龟属Anomala与彩丽金龟属Mimela的亲缘关系较其与弧丽金龟Popillia和喙丽金龟属Adoretus更近。本研究获得了第一条丽金龟亚科最大属——异丽金龟属Anomala昆虫的线粒体基因组全序列,有助于加深对丽金龟亚科线粒体基因组学和系统发育关系的理解。

Anomala corpulenta is an important agricultural and forestry insect pest.In the present study,high-throughput sequencing of A.corpulenta mitochondrial genome was performed using the Illumina HiSeq X platform and the genome sequences were assembled and annotated.Then the structure features and base composition of A.corpulenta mitochondrial genome were analyzed.Based on the nucleotide sequences of 13 protein-coding genes(PCGs),the maximum likelihood and Bayesian methods were used to reconstruct the phylogenetic relationship of Scarabaeidae.The results showed that the complete mitogenome of Anomala corpulenta was 16 673 bp in size,including 13 PCGs,22 tRNA genes,2 rRNA genes and an A+T region with the length of 1 074 bp.The gene arrangement is consistent with those of the known insect species of Rutelinae,and follows the ancestral pattern without gene rearrangement.Phylogenetic analysis showed that all species of Rutelinae belonged to the same branch,confirming Rutelinae to be a monophyly.Genus Anomala was more closely related to Mimela than to Popillia or Adoretus.This is the first report of a complete mitochondrial genome of genus Anomala,the largest genus of Rutelinae,and it will contribute to increasing our knowledge about the mitogenomics and the phylogeny of Rutelinae.