引痰方穴位贴敷涌泉穴对卵清蛋白诱导哮喘小鼠气道炎症的影响

Effect of Acupoint Application at Yongquan Points(KI1)with Yintan Fang on Airway Inflammation in Mice with Ovalbumin-induced Asthma

【目的】观察引痰方穴位贴敷涌泉穴对哮喘小鼠的治疗作用。【方法】从70只雌性BALB/c小鼠中随机选取12只设为正常对照组,其余作为造模组。造模组小鼠采用卵清蛋白(OVA)诱导法构建哮喘模型。将造模成功的48只小鼠随机分为模型对照组、凡士林穴位贴敷组、引痰方穴位贴敷组和布地奈德雾化组,每组12只。连续治疗2周后,显微镜下行支气管肺泡灌洗液(BALF)细胞计数,苏木素-伊红(HE)染色法观察肺组织病理变化,酶联免疫吸附分析(ELISA)检测肺组织匀浆液中白细胞介素(IL)-17、IL-11、IL-13、肿瘤坏死因子(TNF)-α的含量变化。【结果】与正常对照组比较,模型对照组和凡士林穴位贴敷组小鼠BALF中炎症细胞总数和嗜酸性粒细胞数量显著增加(P<0.01),肺组织匀浆液中IL-17、IL-11、IL-13、TNF-α含量显著升高(P<0.05),肺组织可见明显炎症损伤;与模型对照组和凡士林穴位贴敷组比较,引痰方穴位贴敷组和布地奈德雾化组小鼠BALF中炎症细胞总数和嗜酸性粒细胞数量显著下降(P<0.01),肺组织匀浆液中IL-17、IL-11、IL-13、TNF-α含量显著降低(P<0.05或P<0.01),肺组织病理改变明显减轻。引痰方穴位贴敷组上述各指标与布地奈德雾化组比较,差异均无统计学意义(P>0.05)。【结论】引痰方穴位贴敷涌泉穴可抑制哮喘小鼠气道炎症因子释放,减轻炎症反应,控制哮喘发作。

Objective To observe the therapeutic effect of the acupoint application at Yongquan points(KI1)with Yintan Fang for the treatment of the asthma mice.Methods Twelve from 70 female BALB/c mice were randomly selected as the normal control group and the rest as the modeling group.All the mice in the modeling group were constructed to the asthma model by induction of ovalbumin(OVA).The 48 successfully modeled mice were randomly divided into the model control group,Vaseline acupoint application group,Yintan Fang acupoint application group and Budesonide nebulization group,with 12 mice in each group.After 2 weeks of continuous treatment,cell counting of bronchoalveolar lavage fluid(BALF)was performed under microscope,haematoxylineosin(HE)staining was performed to observe the histopathological changes of lung tissue,and enzyme-linked immunosorbent assay(ELISA)was performed to detect the changes in contents of interleukin(IL)-17,IL-11,IL-13 and tumor necrosis factor(TNF)-αin lung tissue homogenate.Results Compared with the normal control group,the total number of inflammatory cells and the number of eosinophils in BALF of mice in the model control group and Vaseline acupoint application group were significantly increased(P<0.01),and the levels of IL-17,IL-11,IL-13 and TNF-αin lung tissue homogenate were significantly increased(P<0.01),and a significant inflammatory injury was seen in lung tissue.Compared with the model control group and the Vaseline acupoint application group,the total number of inflammatory cells and the number of eosinophils in BALF of mice in the Yintan Fang acupoint application group and Budesonide nebulization group were significantly reduced(P<0.01),and the levels of IL-17,IL-11,IL-13 and TNF-αin lung tissue homogenate were significantly reduced(P<0.05 or P<0.01),and the changes of lung histopathology were significantly reduced.None of the above indices were statistically significant in the Yintan Fang acupoint application group compared with the Budesonide nebulization group(P>0.05).Conclusion Acupoint application at Yongquan points(KI1)with Yintan Fang has effects in inhibiting the release of inflammatory factors in the inflamed airways of asthmatic mice,reducing the inflammatory response and controlling asthma attacks.