清肺疏络饮对肺炎支原体肺炎小鼠治疗机制研究

Therapeutic mechanism study on Qingfei Shuluoyin onmycoplasma pneumoniae pneumonia in mice

目的:观察清肺疏络饮对肺炎支原体肺炎小鼠的疗效及其作用机制。方法:选用50只SPF级BALB/c小鼠,按随机数字表法分为正常组、模型组、阿奇霉素组、清肺疏络饮组、联合组,各10只。除正常组外,其余各组小鼠均采用滴鼻接种肺炎支原体菌株造模。造模成功后,阿奇霉素组给予阿奇霉素0.18 ml/20g灌胃,清肺疏络饮组给予清肺疏络饮0.15 ml/20g灌胃,联合组按照上述剂量联合用药,正常组及模型组给予0.9%氯化钠注射液0.20 ml/20 g灌胃,各组小鼠均灌胃7 d。观察肺组织病理变化,检测肺组织内肺炎支原体(MP)水平、P1和E-cd蛋白表达水平。结果:造模后小鼠肺组织镜检符合肺炎改变。灌胃治疗后,阿奇霉素组、清肺疏络饮组小鼠的肺炎改善显著,联合组效果最佳。模型组肺组织P1和E-cd蛋白表达水平较正常组升高,差异有统计学意义(均P<0.05)。治疗后联合组P1和E-cd蛋白表达水平均低于模型组,差异有统计学意义(均P<0.05)。治疗后,清肺疏络饮组、阿奇霉素组小鼠MP均低于模型组,且联合组MP水平最低。结论:清肺疏络饮可以减轻肺炎支原体肺炎小鼠肺部炎症,其作用机制可能与抑制肺组织P1和E-cd蛋白表达,降低MP水平有关。

Objective:To observe the efficacy and mechanism of Qingfei Shuluoyin on mice with mycoplasma pneumoniae pneumonia.Methods:50 BALB/c mice with SPF grade were assigned via the random number table method to the normal group,model group,azithromycin group,Qingfei Shuluoyin group and combinatorial group,each group of 10 mice.Except for the normal group,the other groups were inoculated with mycoplasma pneumoniae strains intranasally to mold.After the success of the model,the azithromycin group were treated with 0.18 ml/20g of azithromycin by intragastric administration,and the dose of Qingfei Shuluoyin reached to 0.15 ml/20g for intragastric administration of the Qingfei Shuluoyin group.The Combinatorial group were simultaneously treated with co-medication of azithromycin and Qingfei Shuluoyin according to their doses mentioned above.Meanwhile,the normal group and the model group were treated with 0.20 ml/20g of 0.9%sodium chloride injection for a total of 7 days.After that,the lung tissue was obtained to observe its pathological changes,and the levels of mycoplasma pneumoniae(MP)and the expression levels of P1 and E-cd proteins in the lung tissue were also measured.Results:The results of microscopic examination in the lung tissue of model mice exhibited the consistency with the changes of pneumonia.After treatment,the pneumonia significantly improved significantly in the azithromycin group and the Qingfei Shuluoyin group,and it possessed the best therapeutic effect in the combinatorial group.The expression levels of P1 and E-cd protein in the lung tissue of the model group were higher than those in the normal group,difference statistically significant(all P<0.05).After treatment,the expression levels of P1 and E-cd protein in the combinatorial group were lower than those in the model group,difference statistically significant(all P<0.05).After treatment,the expression levels of MP in the Qingfei Shuluoyin group and the azithromycin group were lower than those in the model group,and MP in the combinatorial group was the lowest.Conclusion:Qingfei Shuluoyin can reduce lung inflammation in mice with mycoplasma pneumoniae pneumonia,and its mechanism may be related to the inhibition of Qingfei Shuluoyin to P1 and E-cd protein expressions and MP levels in lung tissue.