基于SSR分子标记的象草变异分析

Variation Analysis of Elephant Grass(Pennisetum purpureum) by SSR Molecular Markers

【目的】从分子水平上确认能够在四川泸州叙永县东牛牧场自然越冬并且含糖量有明显差异的7个单株象草材料是否存在遗传变异。【方法】对8份供试材料进行含糖量测定并利用SSR分子标记进行引物筛选及PCR扩增。【结果】22对引物共扩增出166条清晰有效的条带,其中有114条多态性条带,多态性条带比率为68.67%。聚类结果表明,8份供试材料间的遗传相似系数在0.560~0.795之间,SA-4和桂牧1号的遗传相似系数最小,亲缘关系较远。【结论】根据不同引物扩增出现的特异性条带,推测含糖量最高的SA-4是桂牧1号的芽变材料。该结果为象草芽变新材料选育提供了分子生物学依据。

【Objective】At the molecular level,to confirm whether there were genetic variations in seven individual elephant grass materials which could overwinter naturally in Dongniu pasture of Xuyong County,Luzhou City,Sichuan Province and has significant differences in sugar content.【Method】Determination of sugar content in 8 test materials and primer screening and PCR amplification by using SSR molecular markers.【Result】166 clear and effective bands were amplified by 22 pairs of primers,including 114 polymorphic bands,and the polymorphic band ratio was 68.67%.The clustering results showed that the genetic similarity coefficients among the 8 test materials were between 0.560 and 0.795.The genetic similarity coefficient of SA-4 and Guimu-1 was the smallest and the genetic relationship was far.【Conclusion】According to the specific bands amplified by different primers,it was speculated that SA-4 with the highest sugar content was the bud mutation material of Guimu-1.The results will provide a molecular biological basis for the selection of new materials for elephant grass bud mutation.