发酵羊奶对D-半乳糖致衰老模型小鼠皮肤作用的初步探究

Preliminary Study on the Effect of Fermented Goat Milk on the Skin of D-Galactose-Induced Aging Model Mice

【目的】测定酵母菌发酵羊奶的体外总抗氧化能力以及对衰老小鼠皮肤的影响。【方法】选取健康雌性小鼠40只,随机分成4组,空白对照组,衰老模型组,发酵前对照组,发酵实验组。除空白对照组外,其余各组每日颈背部皮下注射D-半乳糖,连续42 d建立衰老模型。造模过程同时对空白对照组与衰老模型组小鼠背部皮肤涂抹无菌水,发酵前对照组涂抹未发酵山羊奶,发酵实验组涂抹发酵后山羊奶。42 d后测定皮肤组织中的水分含量、丙二醛(malonaldehyde,MDA)含量、超氧化物歧化酶(superoxide dismutase,SOD)活性及羟自由基清除能力。【结果】与空白对照组比较,衰老模型组小鼠皮肤水分含量、羟自由基清除能力、SOD酶活力均显著降低(P<0.05),MDA含量升高(P<0.05)。与衰老模型组相比,发酵实验组小鼠皮肤中水分含量、羟自由基清除清除率、SOD酶活力显著升高(P<0.05),MDA含量显著降低(P<0.05)。【结论】发酵羊奶具有一定的延缓皮肤衰老作用。

【Objective】The current study aims to determine the in vitro total antioxidant capacity of yeastfermented goat milk and its effect on the skin of aging mice.【Method】Forty healthy female mice were selected and randomly divided into 4 groups,blank control group,aging model group,pre-fermentation control group,and fermentation experimental group.Except for the blank control group,D-galactose was injected subcutaneously on the nape of the neck every day for 42 consecutive days to establish the aging model.During the modeling process,sterile water was applied to the back skin of the mice in the blank control group and the aging model group,the pre-fermentation control group was applied with unfermented goat milk,and the fermentation experimental group was applied with fermented goat milk.After 42 days,the water content,malonaldehyde(MDA)content,superoxide dismutase(SOD)activity and hydroxyl radical scavenging ability in skin tissue were measured.【Result】Compared with the blank control group,the skin moisture content,hydroxyl radical scavenging ability and SOD enzyme activity of the mice in the aging model group were significantly decreased(P<0.05),and the MDA content was significantly increased(P<0.05).Compared with the aging model group,the water content,hydroxyl radical scavenging rate and SOD enzyme activity in the skin of the mice in the fermentation experimental group were significantly increased(P<0.05),and the MDA content was significantly decreased(P<0.05).【Conclusion】The experiment showed that fermented goat milk has a certain effect on delaying skin aging.