ODF2基因敲减对小鼠精子活力和生育力的影响

Impacts of ODF2 gene knockdown on the sperm motility and fertility of male mice

目的:探讨外周致密纤维蛋白2(ODF2)基因敲减对小鼠精子活力和生育力的影响。方法:构建了3个携ODF2目的基因的基因敲减腺病毒载体及一个空载对照载体。感染ICR小鼠后,通过RT-PCR和Western印迹实验从3个目标载体中确定敲减效果最佳的载体。用此载体感染小鼠,分析基因敲减小鼠多项精子运动参数、睾丸组织病理以及小鼠产仔情况。结果:用敲减效果最佳的载体感染小鼠发现,基因敲减小鼠的精子多项运动参数显著低于正常对照组,睾丸组织生理结构出现异常,产仔数与对照组比较下降明显[(10.86±1.28)只vs(12.72±2.05)只,P=0.001]。结论:ODF2基因表达减少的确会造成小鼠精子多项运动参数异常、睾丸生理结构异常和生育力下降。

Objective: To investigate the knockdown of the outer dense fiber protein 2(ODF2) gene on the sperm motility and fertility of male mice. Methods: We constructed three knockdown vectors with the target gene ODF2 and one control vector without the target gene. After infecting ICR mice, we determined the vector with the best knockdown effect by RT-PCR and Western blot and reinfected the mice with it. Then we obtained and analyzed the sperm motility parameters, pathological changes of the testis issue, and the litter size of the mice with gene knockdown. Results: Compared with the normal controls, the mice infected with the vector with the best knockdown effect showed significantly decreased sperm motility parameters, pathomorphological abnormalities of the testis, and a reduced litter size(10.86 ± 1.28 vs 12.72 ± 2.05, P = 0.001). Conclusion: Decreased expression of the ODF2 gene deceases sperm motility parameters, impairs the morphology of the testis and affects the fertility of male mice.