甘蓝乙二醛酶基因家族的鉴定与表达分析

Identification and Expression Analysis of Glyoxalase Gene Family in Brassica oleracea L.

乙二醛酶(glyoxalase,GLY)是植物应对醛酮类有害物质胁迫的重要酶,对于植物应对非生物胁迫有重要意义。本研究从甘蓝数据库中鉴定获得了15个BolGLYI和16个BolGLYII,利用生物信息学方法系统分析了这些GLY基因的染色体定位、基因结构、蛋白的等电点、分子量、进化关系、保守基序,以及组织表达模式。结果表明,BolGLYI在染色体分布很集中,而BolGLYII的分布比较分散;甘蓝两类GLY基因家族都分成5个亚群,且其与拟南芥的亲缘关系比和水稻更近;同源关系越近的BolGLY基因,其基因结构和保守基序都更相似;BolGLYI家族的基因成员组织表达差异较大,在逆境条件下BolGLYI07和BolGLYI13基因表达量显著高于BolGLYI家族的其他基因,而BolGLYII的各个家族成员在各组织的表达差异不明显,在磷和锌元素胁迫条件下BolGLYII01基因和BolGLYII09基因表达显著高于其他基因,以上结果为进一步研究BolGLY基因的功能提供了理论基础。

Glyoxalase(GLY)is an important enzyme system to deal with the stress of aldehydes and ketones in plants.It is important for plants to respond to abiotic stress.In this study,15 BolGLYI and 16 BolGLYII were identified in Brassica oleracea L.genome database,and their chromosomal location and gene structure,protein isoelectric points,molecular weight,evolutionary relationship,conserved motif,and tissue expression pattern were analyzed by bioinformatics method.The results showed that the distribution of BolGLYI in chromosomes was very concentrated,while the distribution of BolGLYII was scattered.The two BolGLY gene families were divided into five subgroups,and their genetic relationship with Arabidopsis thaliana was closer than that of rice.The GLY genes in the same groups had more similar gene structure and conserved motifs.The expression level among BolGLYI gene members in different tissues were quite different,e.g.,the expression levels of BolGLYI07 and BolGLYI13 genes were significantly higher than those of other BolGLYI genes under stress;while the expression pattern of BolGLYII genes were similar,the expression of BolGLYII01 and BolGLYII09 genes was significantly higher than other genes under stress.The results provide a theoretical basis for further study of the function of BolGLY gene.