基于转录组数据解析大花君子兰花瓣花青素代谢差异

Analyze the Differences in Anthocyanin Metabolism Based on Transcriptome Sequencing of Petal in Clivia miniata

大花君子兰是世界上重要的观赏植物,其花瓣颜色在花蕾不同发育时期具有显著差异。本研究通过对花蕾3个不同发育时期花瓣进行高通量转录组测序,进而探讨君子兰花瓣发育过程中与其花色相关的基因表达情况。结果表明,共获得167078条转录本,平均长度为673 bp,有67512条Unigenes在各数据中被注释。GO功能富集中有24162条基因被注释,分为分子功能、细胞组分和生物过程3大类和50个亚类。KEGG代谢通路注释中,12930条被成功注释,主要富集与花青素合成相关的通路,包括苯丙素生物合成途径、类黄酮生物合成途径、异黄酮生物合成途径、花青素生物合成途径和苯丙氨酸代谢生物合成途径。君子兰花蕾在3个发育时期时花青素合成途径中一些关键酶(如CHS,CHI,DFR)和调控基因(MYB和bHLH)的表达量出现了显著差异。

Clivia miniata is an important ornamental plant in the world.There is significant difference in petal color emerged at different developmental periods of flower bud in C.miniata.In this study,high throughput transcriptome sequencing of the petals at three stages was performed to explore the genes related to the differences in flower color expression of C.miniata.The results showed that 167078 transcripts were obtained,with an average length of 673 bp and 67512 Unigenes were successfully annotated in each data.In GO function enrichment,functional annotation of 24162 genes were divided into three categories and 50 subcategories of molecular function,cell composition and biological process.Among KEGG metabolic pathways,12930 pathways were annotated successfully,and the main enrichment pathways were related to anthocyanin synthesis including phenylpropanoid biosynthesis,flavonoid biosynthesis,isoflavone biosynthesis,anthocyanin biosynthesis and phenylalanine metabolic biosynthesis pathway.Among the key genes expressed in anthocyanin synthesis by KEGG analysis,the expression levels of some key enzymes(such as CHS,CHI and DFR)and regulatory genes(MYB and BHLH)in anthocyanin biosynthesis pathway were significantly different at three developmental stages of flower buds in C.miniata.