博落回提取物对断奶山羊血清代谢组的影响

Effect of Extract of Macleaya cordata on Serum Metabolome of Weaned Goats

为研究博落回(Macleaya cordata)提取物与抗生素导致的山羊(Capra hircas)血清代谢的差异,选取体况相近、遗传背景一致的山羊,随机分为对照组(Control,普通饲粮)、博落回组(Macleaya,普通饲粮+0.3 g/d博落回提取物)和抗生素组[Antibiotics,普通饲粮+21 mg/(kg·d^(–1))万古霉素+42 mg/(kg·d^(–1))新霉素]。结果显示:在饲粮中添加博落回提取物或抗生素对山羊的生长性能没有显著影响(P>0.05),与对照组相比,博落回组血清中白细胞介素-10(IL-10)的含量极显著上升(P<0.01)。另外,α-酮戊二酸在抗生素组与博落回组中的含量均下降。与对照组相比,抗生素组中色氨酸、苯丙氨酸、鹅去氧胆酸盐含量上升,牛磺脱氧胆酸含量下降,博落回组中谷氨酸、亮氨酸含量降低,鸟嘌呤核苷、次黄嘌呤核苷含量上升。KEGG富集分析显示,博落回组与抗生素组的差异代谢物在氨基酸代谢与合成通路上有部分的重合,抗生素组较对照组的差异代谢物富集在苯丙氨酸代谢、氨酰-tRNA生物合成、花生四烯酸代谢、丁酸代谢通路等,而博落回组较对照组的差异代谢物显著富集在氨基酸代谢、谷胱甘肽代谢和嘌呤代谢通路。综上所述,饲粮中添加抗生素或博落回提取物均能改变血清中的氨基酸代谢,而添加博落回提取物可以增强机体的免疫功能,可以替代抗生素在饲料添加剂中的应用。

To research the influence on serum metabolism caused by Macleaya cordat extract and antibiotics in weaning goats (Capra hircas).Goats with similar body condition and consistent genetic background were randomly divided into three treatments.There were the control group (CON group) fed ordinary diet,Macleaya group (MAC group) fed control diet supplemented with 0.3 g/d Macleaya cordata extract and the antibiotics group (ANT group) fed control diet supplemented with21 mg/(kg·d^(–1)) vancomycin and 42 mg/(kg·d^(–1)) neomycin.The results of determination showed that supplementing Macleaya cordata extract or antibiotics did not affect the growth performance (P>0.05).However,compared with the CON group,supplemental Macleaya cordata increased the level of interleukin-10 (IL-10) in the serum (P<0.01).In addition,when compared with the CON group the content of α-ketoglutarate in antibiotics group and MAC group decreased.Antibiotics increased the contents of tryptophan,phenylalanine,chenodeoxycholic,and the content of taurodeoxycholic acid decreased.The contents of glutamic acid and leucine decreased,while the contents of guanosine and inosine increased by Macleaya cordata supplementation.KEGG analysis showed that the differential metabolites of MAC group and ANT group partially coincide in the pathways of amino acid metabolism and synthesis.Compared to CON group,antibiotics treatment affected arachidonic acid metabolism,unsaturated fatty acid biosynthesis and butyric acid metabolism,etc.However,the differential metabolites of MAC group were significantly enriched in amino acid metabolism,glutathione metabolism and purine metabolism pathway compared with the CON group.In conclusion,the addition of antibiotics or Macleaya cordata in the diet can change the amino acid metabolism in the serum,while the addition of Macleaya cordata can enhance the immune function of the body,and can replace antibiotics in the application of feed additives.