迷迭香酸对宫颈癌PI3K/AKT/mTOR信号通路靶分子作用的研究

Effects of Rosmarinic Acid on Target Molecules of PI3K/AKT/mTOR Signaling Pathway in Cervical Cancer

迷迭香酸作为一种天然酚酸类化合物目前已被广泛应用于多种癌症的治疗。为探讨迷迭香酸对人宫颈癌的治疗效果,本研究通过细胞增殖、迁移和自噬等试验进行功能验证,并应用蛋白质免疫印迹技术验证了其可能发挥作用的信号通路。研究结果发现:经迷迭香酸处理后,人宫颈癌Hela细胞的增殖能力显著性下降;细胞的迁移能力同样受到抑制,自噬相关蛋白(ATGs)ATG5和Beclin1(酵母ATG6同源物)表达上调,磷脂酰肌醇-3-激酶(PI3K)、丝氨酸苏氨酸蛋白激酶(AKT)和雷帕霉素靶蛋白(m TOR)表达下降。这提示,迷迭香酸可能通过抑制PI3K/AKT/mTOR信号通路来促进细胞的自噬,并抑制宫颈癌细胞的增殖、迁移。该研究结果进一步说明了迷迭香酸作为抗癌药物的潜在价值,为抗癌药物的研发提供了一个新的理论。

Rosmarinic acid, as a natural acid-secreting compound, has been widely used in the treatment of various cancers. In order to explore the therapeutic effects of rosmarinic acid on human cervical cancer. The experiments of cell proliferation, migration and autophagy were carried out in this study for functional verification, and Western blotting was used to verify the possible signaling pathways. The results showed that the proliferation ability of human cervical cancer significantly decreased after rosmarinic acid treatment;the cell migration ability was also inhibited, the expressions of autophagy-related proteins ATG5 and Beclin1 were upregulated, and the expressions of PI3K, AKT and mTOR proteins decreased. This suggests that rosmarinic acid may promote cell autophagy and inhibit the proliferation and migration of cervical cancer cells by inhibiting the PI3K/AKT/m TOR signaling pathway,thereby achieving the effects of inhibiting or treating human cervical cancer. This further indicates the potential value of rosmarinic acid as an anticancer drug. It provides a new theory for the research and development of anticancer drugs.