皮肤黑色素瘤中EMP3的表达及其与临床预后的相关性分析

The Expression of EMP3 in Skin Cutaneous Melanoma and Its Correlation with Clinical Prognosis

探讨皮肤黑色素瘤(SKCM)中上皮膜蛋白3(EMP3)基因的表达及其与临床预后的相关性。通过GEPIA数据库分析EMP3在SKCM组织与正常组织中的表达水平。通过UALCAN、TIMER和GEPIA数据库分析EMP3表达水平与SKCM患者预后的关系。通过TIMER数据库分析SKCM中EMP3表达与免疫浸润细胞水平、免疫浸润细胞标记物的相关性。结果显示,EMP3在SKCM组织中的表达水平明显高于正常组织。EMP3的表达水平与原发性SKCM患者的总生存率无明显相关性,但是EMP3高表达与转移性SKCM患者较低的总生存率显著相关。B细胞、CD4^(+)T细胞、CD8^(+)T细胞、中性粒细胞、巨噬细胞和树突状细胞的免疫浸润水平与原发性SKCM患者的预后无明显相关性,但是B细胞、CD8^(+)T细胞、中性粒细胞和树突状细胞的低免疫浸润水平与转移性SKCM患者较低的总生存率显著相关。转移性SKCM中EMP3高表达与CD8^(+)T细胞、巨噬细胞和中性粒细胞的低免疫浸润水平及CD8^(+)T细胞、巨噬细胞大部分标记物的低表达显著相关。本研究表明,转移性SKCM中EMP3的表达与免疫细胞浸润及临床预后相关,为转移性SKCM的诊治及预后评估提供了一定的理论基础。

The purpose of this study is to investigate the expression of epithelial membrane protein-3(EMP3) in skin cutaneous melanoma(SKCM) and its correlation with clinical prognosis. The expression of EMP3 in SKCM and normal tissues was analyzed using GEPIA database. UALCAN database, TIMER database and GEPIA database were used to analyze the relationship between the expression of EMP3 and the prognosis of SKCM patients. The correlations between the expression of EMP3and immune infiltration cells level and related markers of immune infiltration cells in SKCM were analyzed using TIMER database. The results showed that the expression of EMP3 in SKCM was significantly higher than that in normal tissues. The expression of EMP3 was not related to the overall survival rate of primary SKCM patients, whereas the high expression of EMP3was significantly related to the lower overall survival rate of metastatic SKCM patients. The infiltration levels of B cells, CD4^(+)T cells, CD8^(+)T cells, neutrophils, macrophages, and dendritic cells were not associated with the prognosis of patients with primary SKCM, whereas the low infiltration levels of B cells, CD8^(+)T cells, neutrophils, and dendritic cells were significantly associated with lower overall survival rate of patients with metastatic SKCM. The high expression of EMP3 in metastatic SKCM was significantly correlated with the lower infiltration levels of CD8^(+)T cells, macrophages and neutrophils, and the lower expression of most markers of CD8^(+)T cells and macrophages. This study suggests that the expression of EMP3 in metastatic SKCM is related to immune infiltration and clinical prognosis, which provides a theoretical basis for the diagnosis, treatment and prognosis evaluation of metastatic SKCM.