环境激素壬基酚对长牡蛎免疫相关基因表达的影响研究

Effects of environmental hormone Nonylphenol stress on the expression of immune-related genes in the Pacific oyster

壬基酚(Nonylphenol,NP)是一种典型的环境内分泌干扰物,具有高亲脂性、难降解性、生物蓄积性和高毒性,其广泛应用引起的环境污染问题越发严重。本研究选取长牡蛎(Crassostrea gigas)为研究对象,关注壬基酚暴露后长牡蛎免疫相关基因的转录表达变化,以探索壬基酚暴露对海洋贝类免疫系统的影响。实验结果表明:长牡蛎的抗氧化酶(SOD、CAT和GPX)、热休克蛋白(Heat shock proteins,HSP)以及NF-κB蛋白家族有关基因在壬基酚刺激后的表达显著提高。实验初步证明了壬基酚刺激可显著影响长牡蛎免疫相关基因的表达,为以后结合病原刺激实验解析贝类大规模死亡原因打下基础;另一方面,也可为明晰海洋贝类壬基酚胁迫响应机制提供参考。

Nonylphenol(NP)is a common environmental endocrine disruptor,which is highly lipophilic,hard to degrade,bioaccumulative and highly toxic.Its widespread application has caused increasingly severe environmental pollution.In this study,Crassostrea gigas was selected as the research object,and the transcriptional expression changes of some immune-related genes in C.gigas after NP exposure were investigated to explore the effect of NP stress on the immune system of marine shellfish.The experimental results showed that the expression levels of genes of antioxidant enzymes(SOD,CAT and GPX),heat shock proteins(HSP)and NF-κB protein family were significantly induced after NP exposure.Our experiments preliminarily proved that NP exposure can significantly affect the expression of immune-related genes in oyster,laying the foundation for the analysis of the causes of mass mortality in shellfish in combination with pathogen stimulation experiments.On the other hand,it also provide a reference for clarifying the response mechanism of NP stress in marine shellfish.