基于网络药理学的仙鹤草-鸡血藤治疗原发免疫性血小板减少症的作用机制研究

Mechanism of action of Agrimonia pilosa-Spatholobi Caulis in treatment of primary immune thrombocytopenia:A study based on network pharmacology

目的:基于网络药理学方法研究仙鹤草-鸡血藤治疗原发免疫性血小板减少症(ITP)的作用机制。方法:通过TCMSP平台筛选仙鹤草-鸡血藤的活性成分及其对应的靶基因,搜索疾病靶点、仙鹤草-鸡血藤治疗ITP的共同靶标基因,构建仙鹤草-鸡血藤活性成分-ITP靶标基因关系网络图、蛋白质-蛋白质相互作用(PPI)网络,进行基因本体(GO)功能和京都基因与基因组百科全书(KEGG)通路富集分析。结果:获得仙鹤草-鸡血藤活性成分29种,作用靶点663个,疾病相关靶点1001个,成分-疾病-靶点47个。PPI网络显示,肿瘤坏死因子、白介素-6、白介素-1β、信号转导及转录激活蛋白3等靶点与仙鹤草-鸡血藤治疗ITP的关系最为密切。GO功能富集分析中,生物过程主要集中在对炎症反应、细胞对有机化合物的反应、对脂多糖的反应等;分子功能主要集中在蛋白结合、细胞因子活性、相同的蛋白质结合等;细胞组分主要集中在细胞外间隙、等离子膜外侧、细胞外区域等。KEGG通路富集分析主要包括肿瘤坏死因子信号通路、趋化因子信号通路、Toll样受体信号通路、磷脂酰肌醇3-激酶-丝氨酸-苏氨酸激酶信号通路等。结论:仙鹤草-鸡血藤可通过多靶标、多途径发挥治疗ITP的作用。

Objective:To investigate the mechanism of action of Agrimonia pilosa-Spatholobi Caulis in the treatment of primary immune thrombocytopenia(ITP)based on network pharmacology.Methods:The TCMSP platform was used to screen out the active components of Agrimonia pilosa-Spatholobi Caulis and their corresponding target genes,and the common target genes of the disease targets and the target genes of Agrimonia pilosa-Spatholobi Caulis in the treatment of ITP were obtained to construct a network of Agrimonia pilosa-Spatholobi Caulis active components and ITP target genes and a protein-protein interaction(PPI)network.Gene ontology(GO)functional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis were also performed.Results:A total of 29 active components and 663 action targets were obtained for Agrimonia pilosa-Spatholobi Caulis,and there were 1001 disease targets and 47 component-disease-target common targets.PPI network showed that the targets such as tumor necrosis factor,interleukin-6,interleukin-1β,signal transduction,transcription activating protein 3 were closely associated with Agrimonia pilosa-Spatholobi Caulis in the treatment of ITP.GO functional enrichment analysis showed that the main biological processes included inflammatory response,response of cells to organic compounds,and response to lipopolysaccharide;the main molecular functions included protein binding,cytokine activity,and binding of the same proteins;cellular components were mainly found in extracellular space,outer plasma membrane,and extracellular domain.KEGG pathway enrichment analysis showed that the pathways mainly included the tumor necrosis factor signaling pathway,the chemokine signaling pathway,the Toll-like receptor signaling pathway,and the phosphatidylinositol 3-kinase/serine-threonine kinase signaling pathway.Conclusion:Agrimonia pilosa-Spatholobi Caulis exerts a therapeutic effect on ITP via multiple targets and pathways.