观音座莲的抗炎止血活性及其化学成分研究

ANTI-INFLAMMATORY AND HEMOSTATIC ACTIVITIES AND CHEMICAL CONSTITUENTS OF ANGIOPTERIS FOKIENSIS

探讨观音座莲的抗炎、止血作用及其化学成分。利用二甲苯致小鼠耳肿胀、蛋清致小鼠足趾肿胀模型,观察观音座莲提取物的抗炎作用;采用小鼠断尾止血及玻片法观察观音座莲提取物对小鼠出血时间、血液出现血丝时间的影响;利用化学及波谱方法明确其化学成分。结果显示:观音座莲提取物可显著抑制二甲苯引起小鼠耳肿胀和蛋清致小鼠足趾肿胀,明显缩短小鼠断尾出血时间和血液出现血丝时间,从其提取物中得到芹菜素、木犀草素两种黄酮类化合物和β-谷甾醇一种甾体成分。观音座莲具有较好的抗炎、止血作用,化学成分主要包括黄酮类及甾体类等。

To approach the anti-inflammatory,hemostasis effects of the extracts and the chemical constituents of Angiopteris fokiensis.The anti-inflammatory effects were observed on the models of xylene-induced ears swelling and albumin induced mouse paw edema.The effects of extract against clotting and bleeding of the mice were observed with the slide method and severed tail.Various chromatographic volumes were used to separate and purify the constituents.The extracts of A.fokiensis could obviously inhibit ears and foots swelling and decrease the bleeding time of the mice with the severed tail and clotting time.Three compounds apigenin,luteolin,β-sitosterol were obtained.The extracts of A.fokiensis have better anti-inflammatory,hemostasis effects.The main compounds cover flavonoids,steroids.