自动核酸提取仪校准结果不确定度评定

Evaluation of Uncertainty in Calibration Results for Automatic Nucleic Acid Extractors

自动核酸提取仪采用核酸提取试剂完成对样本核酸的提取,在新冠疫情期间,广泛应用于核酸检测实验,其质量直接影响样品核酸提取效率和检测结果的准确性。本文介绍了自动核酸提取仪的定义、结构原理和作用,并对其温度、振动频率和核酸提取回收率进行不确定度评定和分析。

The automatic nucleic acid extractor uses nucleic acid extraction reagent to complete nucleic acid extraction of samples.It is widely used in nucleic acid detection experiments during the COVID-19 pandemic.Its quality directly affects the efficiency of nucleic acid extraction of samples and the accuracy of test results.This paper introduces the definition,structure principle and function of automatic nucleic acid extractors,and evaluates and analyzes the uncertainty of its temperature,vibration frequency and recovery rate of nucleic acid extraction.