LINC01615表达下调对膀胱癌细胞增殖、侵袭、迁移的影响

Effects of down-regulated expression of LINC01615 on proliferation,invasion and migration of bladder cancer cells

目的 探讨下调LINC01615表达对膀胱癌细胞(T24细胞)增殖、侵袭、迁移的影响。方法 常规培养T24细胞,取对数生长期细胞随机分为si-LINC01615组、si-NC组,分别转染si-LINC01615、si-NC,继续培养48 h。用实时荧光定量PCR法检测细胞中LINC01615表达,用MTT实验检测细胞增殖能力(OD值),用Transwell实验检测细胞侵袭能力,用划痕实验检测细胞迁移能力(24 h愈合度)。结果 转染后si-LINC01615组、si-NC组T24细胞LINC01615相对表达量分别为0.60±0.03、1.08±0.02,比较差异有统计学意义(P<0.05),表明转染成功。培养0、24 h,两组细胞活力差异均无统计学意义(P均>0.05);培养48、72 h,si-LINC01615组细胞活力均低于si-NC组(P均<0.05)。si-NC组侵袭细胞数为(148.30±1.76)个,si-LINC01615组为(74.33±3.18)个,比较差异有统计学意义(P<0.05)。si-NC组24 h愈合度为(98.33±0.88)%,si-LINC01615组为(62.19±0.99)%,比较差异有统计学意义(P<0.05)。结论 下调LINC01615表达可抑制膀胱癌细胞增殖、侵袭、迁移。

Objective To investigate the effects of down-regulated expression of LINC01615 on the proliferation, invasion, and migration of bladder cancer cells(T24 cells). Methods T24 cells were cultured conventionally. Cells in the logarithmic phase were randomly divided into the si-LINC01615 group and si-NC group, which were transfected with siLINC01615 and si-NC, respectively, followed by continued culture of 48 h. The expression of LINC01615 in cells was detected by real-time fluorescence quantitative PCR, cell proliferation ability(OD value) was detected by MTT assay, cell invasion ability was detected by Transwell assay, and cell migration ability(24 h healing degree) was detected by scratch assay. Results After transfection, the relative expression levels of LINC01615 in T24 cells in the si-LINC01615 group and si-NC group were 0. 60±0. 03 and 1. 08±0. 02, respectively, with statistical significance(P<0. 05), indicating successful transfection. At 0 and 24 h, there was no statistically significant difference in the cell viability between the two groups(all P>0. 05). At 48 and 72 h, the cell viability of the si-LINC01615 group was lower than that of the si-NC group(P<0. 05). The number of invasive cells was 148. 30±1. 76 in the si-NC group and(74. 33±3. 18) in the si-LINC01615group, and the difference was statistically significant(P<0. 05). The 24 h healing degree was 98. 33%±0. 88% in the siNC group and 62. 19%±0. 99 % in the si-LINC01615 group, and the difference was statistically significant(P<0. 05).Conclusion Down-regulation of LINC01615 expression can inhibit the proliferation, invasion and migration of bladder cancer cells.